Water-soluble conjugate of double-stranded DNA and poly(N-isopropylacrylamide) for one-pot affinity precipitation separation of DNA-binding proteins

Daisuke Umeno, Masafumi Kawasaki, Mizuo Maeda*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

Poly(N-isopropylacrylamide) having a terminal psoralen group was synthesized and covalently bound to plasmid pBR 322 DNA through a photochemical reaction. The resulting conjugate exhibited temperature-responsive precipitation due to the aggregation of poly(N-isopropylacrylamide) chains when heated above 31 °C. This system was used for the one-pot separation of restriction endonuclease EcoRI.

Original languageEnglish
Pages (from-to)719-724
Number of pages6
JournalBioconjugate Chemistry
Volume9
Issue number6
DOIs
Publication statusPublished - 1998 Nov
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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