Whole Genome Analyses of Marine Fish Pathogenic Isolate, Mycobacterium sp. 012931

Satoru Kurokawa, Jun Kabayama, Seong Don Hwang, Seong Won Nho, Jun ichi Hikima, Tae Sung Jung, Hidehiro Kondo, Ikuo Hirono, Haruko Takeyama, Tetsushi Mori, Takashi Aoki

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Mycobacterium is a genus within the order Actinomycetales that comprises of a large number of well-characterized species, several of which includes pathogens known to cause serious disease in human and animal. Here, we report the whole genome sequence of Mycobacterium sp. strain 012931 isolated from the marine fish, yellowtail (Seriola quinqueradiata). Mycobacterium sp. 012931 is a fish pathogen causing serious damage to aquaculture farms in Japan. DNA dot plot analysis showed that Mycobacterium sp. 012931 was more closely related to Mycobacterium marinum when compared across several Mycobacterium species. However, little conservation of the gene order was observed between Mycobacterium sp. 012931 and M. marinum genome. The annotated 5,464 genes of Mycobacterium sp. 012931 was classified into 26 subsystems. The insertion/deletion gene analysis shows Mycobacterium sp. 012931 had 643 unique genes that were not found in the M. marinum strains. In the virulence, disease, and defense subsystem, both insertion and deletion genes of Mycobacterium sp. 012931 were associated with the PPE gene cluster of Mycobacteria. Of seven plcB genes in Mycobacterium sp. 012931, plcB_2 and plcB_3 showed low identities with those of M. marinum strains. Therefore, Mycobacterium sp. 012931 has differences on genetic and virulence from M. marinum and may induce different interaction mechanisms between host and pathogen.

    Original languageEnglish
    Pages (from-to)572-579
    Number of pages8
    JournalMarine Biotechnology
    Volume16
    Issue number5
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    Mycobacterium
    marine fish
    genome
    Genome
    Mycobacterium marinum
    gene
    fish
    pathogen
    virulence
    gene deletion
    Insertional Mutagenesis
    Gene Deletion
    Virulence
    pathogens
    Fishes
    genes
    Actinomycetales
    aquaculture
    aquaculture farms
    Seriola quinqueradiata

    Keywords

    • Fish pathogen
    • M. marinum
    • Mycobacterium sp.
    • PPE gene cluster
    • Whole genome analysis

    ASJC Scopus subject areas

    • Aquatic Science
    • Biotechnology

    Cite this

    Kurokawa, S., Kabayama, J., Hwang, S. D., Nho, S. W., Hikima, J. I., Jung, T. S., ... Aoki, T. (2014). Whole Genome Analyses of Marine Fish Pathogenic Isolate, Mycobacterium sp. 012931. Marine Biotechnology, 16(5), 572-579. https://doi.org/10.1007/s10126-014-9576-x

    Whole Genome Analyses of Marine Fish Pathogenic Isolate, Mycobacterium sp. 012931. / Kurokawa, Satoru; Kabayama, Jun; Hwang, Seong Don; Nho, Seong Won; Hikima, Jun ichi; Jung, Tae Sung; Kondo, Hidehiro; Hirono, Ikuo; Takeyama, Haruko; Mori, Tetsushi; Aoki, Takashi.

    In: Marine Biotechnology, Vol. 16, No. 5, 2014, p. 572-579.

    Research output: Contribution to journalArticle

    Kurokawa, S, Kabayama, J, Hwang, SD, Nho, SW, Hikima, JI, Jung, TS, Kondo, H, Hirono, I, Takeyama, H, Mori, T & Aoki, T 2014, 'Whole Genome Analyses of Marine Fish Pathogenic Isolate, Mycobacterium sp. 012931', Marine Biotechnology, vol. 16, no. 5, pp. 572-579. https://doi.org/10.1007/s10126-014-9576-x
    Kurokawa, Satoru ; Kabayama, Jun ; Hwang, Seong Don ; Nho, Seong Won ; Hikima, Jun ichi ; Jung, Tae Sung ; Kondo, Hidehiro ; Hirono, Ikuo ; Takeyama, Haruko ; Mori, Tetsushi ; Aoki, Takashi. / Whole Genome Analyses of Marine Fish Pathogenic Isolate, Mycobacterium sp. 012931. In: Marine Biotechnology. 2014 ; Vol. 16, No. 5. pp. 572-579.
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