Whole genome sequence of Desulfovibrio magneticus strain RS-1 revealed common gene clusters in magnetotactic bacteria

Hidekazu Nakazawa, Atsushi Arakaki, Sachiko Narita-Yamada, Isao Yashiro, Koji Jinno, Natsuko Aoki, Ai Tsuruyama, Yoshiko Okamura, Satoshi Tanikawa, Nobuyuki Fujita, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Magnetotactic bacteria are ubiquitous microorganisms that synthesize intracellular magnetite particles (magnetosomes) by accumulating Fe ions from aquatic environments. Recent molecular studies, including comprehensive proteomic, transcriptomic, and genomic analyses, have considerably improved our hypotheses of the magnetosome-formation mechanism. However, most of these studies have been conducted using pure-cultured bacterial strains of α-proteobacteria. Here, we report the whole-genome sequence of Desulfovibrio magneticus strain RS-1, the only isolate of magnetotactic microorganisms classified under d-proteobacteria. Comparative genomics of the RS-1 and four a-proteobacterial strains revealed the presence of three separate gene regions (nuo and mamAB-like gene clusters, and gene region of a cryptic plasmid) conserved in all magnetotactic bacteria. The nuo gene cluster, encoding NADH dehydrogenase (complex I), was also common to the genomes of three iron-reducing bacteria exhibiting uncontrolled extracellular and/or intracellular magnetite synthesis. A cryptic plasmid, pDMC1, encodes three homologous genes that exhibit high similarities with those of other magnetotactic bacterial strains. In addition, the mamAB-like gene cluster, encoding the key components for magnetosome formation such as iron transport and magnetosome alignment, was conserved only in the genomes of magnetotactic bacteria as a similar genomic island-like structure. Our findings suggest the presence of core genetic components for magnetosome biosynthesis; these genes may have been acquired into the magnetotactic bacterial genomes by multiple gene-transfer events during proteobacterial evolution.

Original languageEnglish
Pages (from-to)1801-1808
Number of pages8
JournalGenome Research
Volume19
Issue number10
DOIs
Publication statusPublished - 2009 Oct
Externally publishedYes

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Magnetosomes
Desulfovibrio
Multigene Family
Genome
Bacteria
Ferrosoferric Oxide
Proteobacteria
Genes
Plasmids
Iron
Electron Transport Complex I
Bacterial Genomes
Genomic Islands
Genomics
Proteomics
Ions

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Nakazawa, H., Arakaki, A., Narita-Yamada, S., Yashiro, I., Jinno, K., Aoki, N., ... Matsunaga, T. (2009). Whole genome sequence of Desulfovibrio magneticus strain RS-1 revealed common gene clusters in magnetotactic bacteria. Genome Research, 19(10), 1801-1808. https://doi.org/10.1101/gr.088906.108

Whole genome sequence of Desulfovibrio magneticus strain RS-1 revealed common gene clusters in magnetotactic bacteria. / Nakazawa, Hidekazu; Arakaki, Atsushi; Narita-Yamada, Sachiko; Yashiro, Isao; Jinno, Koji; Aoki, Natsuko; Tsuruyama, Ai; Okamura, Yoshiko; Tanikawa, Satoshi; Fujita, Nobuyuki; Takeyama, Haruko; Matsunaga, Tadashi.

In: Genome Research, Vol. 19, No. 10, 10.2009, p. 1801-1808.

Research output: Contribution to journalArticle

Nakazawa, H, Arakaki, A, Narita-Yamada, S, Yashiro, I, Jinno, K, Aoki, N, Tsuruyama, A, Okamura, Y, Tanikawa, S, Fujita, N, Takeyama, H & Matsunaga, T 2009, 'Whole genome sequence of Desulfovibrio magneticus strain RS-1 revealed common gene clusters in magnetotactic bacteria', Genome Research, vol. 19, no. 10, pp. 1801-1808. https://doi.org/10.1101/gr.088906.108
Nakazawa, Hidekazu ; Arakaki, Atsushi ; Narita-Yamada, Sachiko ; Yashiro, Isao ; Jinno, Koji ; Aoki, Natsuko ; Tsuruyama, Ai ; Okamura, Yoshiko ; Tanikawa, Satoshi ; Fujita, Nobuyuki ; Takeyama, Haruko ; Matsunaga, Tadashi. / Whole genome sequence of Desulfovibrio magneticus strain RS-1 revealed common gene clusters in magnetotactic bacteria. In: Genome Research. 2009 ; Vol. 19, No. 10. pp. 1801-1808.
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