Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection

Makoto Kuroda, Atsushi Yamashita, Hideki Hirakawa, Miyuki Kumano, Kazuya Morikawa, Masato Higashide, Atsushi Maruyama, Yumiko Inose, Kimio Matoba, Hidehiro Toh, Satoru Kuhara, Masahira Hattori, Toshiko Ohta

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

Staphylococcus saprophyticus is a uropathogenic Staphylococcus frequently isolated from young female outpatients presenting with uncomplicated urinary tract infections. We sequenced the whole genome of S. saprophyticus type strain ATCC 15305, which harbors a circular chromosome of 2,516,575 bp with 2,446 ORFs and two plasmids. Comparative genomic analyses with the strains of two other species, Staphylococcus aureus and Staphylococcus epidermidis, as well as experimental data, revealed the following characteristics of the S. saprophyticus genome. S. saprophyticus does not possess any virulence factors found in S. aureus, such as coagulase, enterotoxins, exoenzymes, and extracellular matrix-binding proteins, although it does have a remarkable paralog expansion of transport systems related to highly variable ion contents in the urinary environment. A further unique feature is that only a single ORF is predictable as a cell wall-anchored protein, and it shows positive hemagglutination and adherence to human bladder cell associated with initial colonization in the urinary tract. It also shows significantly high urease activity in S. saprophyticus. The uropathogenicity of S. saprophyticus can be attributed to its genome that is needed for its survival in the human urinary tract by means of novel cell wall-anchored adhesin and redundant uro-adaptive transport systems, together with urease.

Original languageEnglish
Pages (from-to)13272-13277
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number37
DOIs
Publication statusPublished - 2005 Sep 13
Externally publishedYes

Fingerprint

Staphylococcus saprophyticus
Urinary Tract Infections
Genome
Urease
Urinary Tract
Cell Wall
Open Reading Frames
Staphylococcus aureus
Chromosomes, Human, Pair 2
Staphylococcus epidermidis
Extracellular Matrix Proteins
Coagulase
Enterotoxins
Hemagglutination
Virulence Factors
Staphylococcus
Carrier Proteins
Urinary Bladder
Plasmids
Outpatients

Keywords

  • Capsule
  • Divalent-cation transporter
  • Hemagglutination
  • Paralog expansion
  • Staphylococcal cassette chromosome

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection. / Kuroda, Makoto; Yamashita, Atsushi; Hirakawa, Hideki; Kumano, Miyuki; Morikawa, Kazuya; Higashide, Masato; Maruyama, Atsushi; Inose, Yumiko; Matoba, Kimio; Toh, Hidehiro; Kuhara, Satoru; Hattori, Masahira; Ohta, Toshiko.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 37, 13.09.2005, p. 13272-13277.

Research output: Contribution to journalArticle

Kuroda, M, Yamashita, A, Hirakawa, H, Kumano, M, Morikawa, K, Higashide, M, Maruyama, A, Inose, Y, Matoba, K, Toh, H, Kuhara, S, Hattori, M & Ohta, T 2005, 'Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 37, pp. 13272-13277. https://doi.org/10.1073/pnas.0502950102
Kuroda, Makoto ; Yamashita, Atsushi ; Hirakawa, Hideki ; Kumano, Miyuki ; Morikawa, Kazuya ; Higashide, Masato ; Maruyama, Atsushi ; Inose, Yumiko ; Matoba, Kimio ; Toh, Hidehiro ; Kuhara, Satoru ; Hattori, Masahira ; Ohta, Toshiko. / Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 37. pp. 13272-13277.
@article{6dee97af1174460e91450162377cd7c9,
title = "Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection",
abstract = "Staphylococcus saprophyticus is a uropathogenic Staphylococcus frequently isolated from young female outpatients presenting with uncomplicated urinary tract infections. We sequenced the whole genome of S. saprophyticus type strain ATCC 15305, which harbors a circular chromosome of 2,516,575 bp with 2,446 ORFs and two plasmids. Comparative genomic analyses with the strains of two other species, Staphylococcus aureus and Staphylococcus epidermidis, as well as experimental data, revealed the following characteristics of the S. saprophyticus genome. S. saprophyticus does not possess any virulence factors found in S. aureus, such as coagulase, enterotoxins, exoenzymes, and extracellular matrix-binding proteins, although it does have a remarkable paralog expansion of transport systems related to highly variable ion contents in the urinary environment. A further unique feature is that only a single ORF is predictable as a cell wall-anchored protein, and it shows positive hemagglutination and adherence to human bladder cell associated with initial colonization in the urinary tract. It also shows significantly high urease activity in S. saprophyticus. The uropathogenicity of S. saprophyticus can be attributed to its genome that is needed for its survival in the human urinary tract by means of novel cell wall-anchored adhesin and redundant uro-adaptive transport systems, together with urease.",
keywords = "Capsule, Divalent-cation transporter, Hemagglutination, Paralog expansion, Staphylococcal cassette chromosome",
author = "Makoto Kuroda and Atsushi Yamashita and Hideki Hirakawa and Miyuki Kumano and Kazuya Morikawa and Masato Higashide and Atsushi Maruyama and Yumiko Inose and Kimio Matoba and Hidehiro Toh and Satoru Kuhara and Masahira Hattori and Toshiko Ohta",
year = "2005",
month = "9",
day = "13",
doi = "10.1073/pnas.0502950102",
language = "English",
volume = "102",
pages = "13272--13277",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "37",

}

TY - JOUR

T1 - Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection

AU - Kuroda, Makoto

AU - Yamashita, Atsushi

AU - Hirakawa, Hideki

AU - Kumano, Miyuki

AU - Morikawa, Kazuya

AU - Higashide, Masato

AU - Maruyama, Atsushi

AU - Inose, Yumiko

AU - Matoba, Kimio

AU - Toh, Hidehiro

AU - Kuhara, Satoru

AU - Hattori, Masahira

AU - Ohta, Toshiko

PY - 2005/9/13

Y1 - 2005/9/13

N2 - Staphylococcus saprophyticus is a uropathogenic Staphylococcus frequently isolated from young female outpatients presenting with uncomplicated urinary tract infections. We sequenced the whole genome of S. saprophyticus type strain ATCC 15305, which harbors a circular chromosome of 2,516,575 bp with 2,446 ORFs and two plasmids. Comparative genomic analyses with the strains of two other species, Staphylococcus aureus and Staphylococcus epidermidis, as well as experimental data, revealed the following characteristics of the S. saprophyticus genome. S. saprophyticus does not possess any virulence factors found in S. aureus, such as coagulase, enterotoxins, exoenzymes, and extracellular matrix-binding proteins, although it does have a remarkable paralog expansion of transport systems related to highly variable ion contents in the urinary environment. A further unique feature is that only a single ORF is predictable as a cell wall-anchored protein, and it shows positive hemagglutination and adherence to human bladder cell associated with initial colonization in the urinary tract. It also shows significantly high urease activity in S. saprophyticus. The uropathogenicity of S. saprophyticus can be attributed to its genome that is needed for its survival in the human urinary tract by means of novel cell wall-anchored adhesin and redundant uro-adaptive transport systems, together with urease.

AB - Staphylococcus saprophyticus is a uropathogenic Staphylococcus frequently isolated from young female outpatients presenting with uncomplicated urinary tract infections. We sequenced the whole genome of S. saprophyticus type strain ATCC 15305, which harbors a circular chromosome of 2,516,575 bp with 2,446 ORFs and two plasmids. Comparative genomic analyses with the strains of two other species, Staphylococcus aureus and Staphylococcus epidermidis, as well as experimental data, revealed the following characteristics of the S. saprophyticus genome. S. saprophyticus does not possess any virulence factors found in S. aureus, such as coagulase, enterotoxins, exoenzymes, and extracellular matrix-binding proteins, although it does have a remarkable paralog expansion of transport systems related to highly variable ion contents in the urinary environment. A further unique feature is that only a single ORF is predictable as a cell wall-anchored protein, and it shows positive hemagglutination and adherence to human bladder cell associated with initial colonization in the urinary tract. It also shows significantly high urease activity in S. saprophyticus. The uropathogenicity of S. saprophyticus can be attributed to its genome that is needed for its survival in the human urinary tract by means of novel cell wall-anchored adhesin and redundant uro-adaptive transport systems, together with urease.

KW - Capsule

KW - Divalent-cation transporter

KW - Hemagglutination

KW - Paralog expansion

KW - Staphylococcal cassette chromosome

UR - http://www.scopus.com/inward/record.url?scp=24944592355&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24944592355&partnerID=8YFLogxK

U2 - 10.1073/pnas.0502950102

DO - 10.1073/pnas.0502950102

M3 - Article

VL - 102

SP - 13272

EP - 13277

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 37

ER -