Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection

Makoto Kuroda, Atsushi Yamashita, Hideki Hirakawa, Miyuki Kumano, Kazuya Morikawa, Masato Higashide, Atsushi Maruyama, Yumiko Inose, Kimio Matoba, Hidehiro Toh, Satoru Kuhara, Masahira Hattori, Toshiko Ohta

Research output: Contribution to journalArticle

169 Citations (Scopus)

Abstract

Staphylococcus saprophyticus is a uropathogenic Staphylococcus frequently isolated from young female outpatients presenting with uncomplicated urinary tract infections. We sequenced the whole genome of S. saprophyticus type strain ATCC 15305, which harbors a circular chromosome of 2,516,575 bp with 2,446 ORFs and two plasmids. Comparative genomic analyses with the strains of two other species, Staphylococcus aureus and Staphylococcus epidermidis, as well as experimental data, revealed the following characteristics of the S. saprophyticus genome. S. saprophyticus does not possess any virulence factors found in S. aureus, such as coagulase, enterotoxins, exoenzymes, and extracellular matrix-binding proteins, although it does have a remarkable paralog expansion of transport systems related to highly variable ion contents in the urinary environment. A further unique feature is that only a single ORF is predictable as a cell wall-anchored protein, and it shows positive hemagglutination and adherence to human bladder cell associated with initial colonization in the urinary tract. It also shows significantly high urease activity in S. saprophyticus. The uropathogenicity of S. saprophyticus can be attributed to its genome that is needed for its survival in the human urinary tract by means of novel cell wall-anchored adhesin and redundant uro-adaptive transport systems, together with urease.

Original languageEnglish
Pages (from-to)13272-13277
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number37
DOIs
Publication statusPublished - 2005 Sep 13
Externally publishedYes

Keywords

  • Capsule
  • Divalent-cation transporter
  • Hemagglutination
  • Paralog expansion
  • Staphylococcal cassette chromosome

ASJC Scopus subject areas

  • Genetics
  • General

Fingerprint Dive into the research topics of 'Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection'. Together they form a unique fingerprint.

  • Cite this

    Kuroda, M., Yamashita, A., Hirakawa, H., Kumano, M., Morikawa, K., Higashide, M., Maruyama, A., Inose, Y., Matoba, K., Toh, H., Kuhara, S., Hattori, M., & Ohta, T. (2005). Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection. Proceedings of the National Academy of Sciences of the United States of America, 102(37), 13272-13277. https://doi.org/10.1073/pnas.0502950102