Widefield real-time single-cell secretion imaging with optical waveguide technique

Yumiko Tanaka, Nobutake Suzuki, Kazuyo Mora, Jun Mizuno, Shuichi Shoji, Sotaro Uemura, Yoshitaka Shirasaki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report the novel methodology which enables us to analyze single-cell secretion dynamics on the widefield total internal reflection fluorescence imaging with high spatial and temporal resolution using the optical waveguide technique. The practical system was achieved after some sensitivity improvements in an optical waveguide imaging, which has succeeded in detecting very weak fluorescence signals reflecting single-cell secretion dynamics in spite of using a low magnification objective lens. Our methodology is more advantageous than other traditional microscopic technologies in real-time imaging of cell-to-cell communication through humoral factor secretion among actively migrating cells, like immune cells do in vivo.

Original languageEnglish
Title of host publicationTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1580-1583
Number of pages4
ISBN (Electronic)9781538627310
DOIs
Publication statusPublished - 2017 Jul 26
Event19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China
Duration: 2017 Jun 182017 Jun 22

Other

Other19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
CountryTaiwan, Province of China
CityKaohsiung
Period17/6/1817/6/22

Fingerprint

secretions
Optical waveguides
optical waveguides
Imaging techniques
cells
Fluorescence
Lenses
methodology
fluorescence
Communication
magnification
temporal resolution
spatial resolution
communication
lenses
sensitivity
high resolution

Keywords

  • live cell imaging
  • optical waveguide
  • secretion
  • Single cell

ASJC Scopus subject areas

  • Chemical Health and Safety
  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Tanaka, Y., Suzuki, N., Mora, K., Mizuno, J., Shoji, S., Uemura, S., & Shirasaki, Y. (2017). Widefield real-time single-cell secretion imaging with optical waveguide technique. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 1580-1583). [7994363] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994363

Widefield real-time single-cell secretion imaging with optical waveguide technique. / Tanaka, Yumiko; Suzuki, Nobutake; Mora, Kazuyo; Mizuno, Jun; Shoji, Shuichi; Uemura, Sotaro; Shirasaki, Yoshitaka.

TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. p. 1580-1583 7994363.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tanaka, Y, Suzuki, N, Mora, K, Mizuno, J, Shoji, S, Uemura, S & Shirasaki, Y 2017, Widefield real-time single-cell secretion imaging with optical waveguide technique. in TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems., 7994363, Institute of Electrical and Electronics Engineers Inc., pp. 1580-1583, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, Kaohsiung, Taiwan, Province of China, 17/6/18. https://doi.org/10.1109/TRANSDUCERS.2017.7994363
Tanaka Y, Suzuki N, Mora K, Mizuno J, Shoji S, Uemura S et al. Widefield real-time single-cell secretion imaging with optical waveguide technique. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc. 2017. p. 1580-1583. 7994363 https://doi.org/10.1109/TRANSDUCERS.2017.7994363
Tanaka, Yumiko ; Suzuki, Nobutake ; Mora, Kazuyo ; Mizuno, Jun ; Shoji, Shuichi ; Uemura, Sotaro ; Shirasaki, Yoshitaka. / Widefield real-time single-cell secretion imaging with optical waveguide technique. TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 1580-1583
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