Widefield real-time single-cell secretion imaging with optical waveguide technique

Yumiko Tanaka; Nobutake Suzuki; Kazuyo Mora; Jun Mizuno; Shuichi Shoji; Sotaro Uemura; Yoshitaka Shirasaki

doi:10.1109/TRANSDUCERS.2017.7994363

Widefield real-time single-cell secretion imaging with optical waveguide technique

Yumiko Tanaka, Nobutake Suzuki, Kazuyo Mora, Jun Mizuno, Shuichi Shoji, Sotaro Uemura, Yoshitaka Shirasaki^*

^*Corresponding author for this work

School of Fundamental Science and Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

We report the novel methodology which enables us to analyze single-cell secretion dynamics on the widefield total internal reflection fluorescence imaging with high spatial and temporal resolution using the optical waveguide technique. The practical system was achieved after some sensitivity improvements in an optical waveguide imaging, which has succeeded in detecting very weak fluorescence signals reflecting single-cell secretion dynamics in spite of using a low magnification objective lens. Our methodology is more advantageous than other traditional microscopic technologies in real-time imaging of cell-to-cell communication through humoral factor secretion among actively migrating cells, like immune cells do in vivo.

Original language	English
Title of host publication	TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	1580-1583
Number of pages	4
ISBN (Electronic)	9781538627310
DOIs	https://doi.org/10.1109/TRANSDUCERS.2017.7994363
Publication status	Published - 2017 Jul 26
Event	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China Duration: 2017 Jun 18 → 2017 Jun 22

Publication series

Name	TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
Country/Territory	Taiwan, Province of China
City	Kaohsiung
Period	17/6/18 → 17/6/22

Keywords

Single cell
live cell imaging
optical waveguide
secretion

ASJC Scopus subject areas

Chemical Health and Safety
Instrumentation
Electrical and Electronic Engineering

Access to Document

10.1109/TRANSDUCERS.2017.7994363

Cite this

Tanaka, Y., Suzuki, N., Mora, K., Mizuno, J., Shoji, S., Uemura, S., & Shirasaki, Y. (2017). Widefield real-time single-cell secretion imaging with optical waveguide technique. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 1580-1583). [7994363] (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994363

Widefield real-time single-cell secretion imaging with optical waveguide technique. / Tanaka, Yumiko; Suzuki, Nobutake; Mora, Kazuyo et al.

TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. p. 1580-1583 7994363 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Tanaka, Y, Suzuki, N, Mora, K, Mizuno, J, Shoji, S, Uemura, S & Shirasaki, Y 2017, Widefield real-time single-cell secretion imaging with optical waveguide technique. in TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems., 7994363, TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, Institute of Electrical and Electronics Engineers Inc., pp. 1580-1583, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, Kaohsiung, Taiwan, Province of China, 17/6/18. https://doi.org/10.1109/TRANSDUCERS.2017.7994363

Tanaka Y, Suzuki N, Mora K, Mizuno J, Shoji S, Uemura S et al. Widefield real-time single-cell secretion imaging with optical waveguide technique. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc. 2017. p. 1580-1583. 7994363. (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). doi: 10.1109/TRANSDUCERS.2017.7994363

Tanaka, Yumiko ; Suzuki, Nobutake ; Mora, Kazuyo et al. / Widefield real-time single-cell secretion imaging with optical waveguide technique. TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 1580-1583 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

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abstract = "We report the novel methodology which enables us to analyze single-cell secretion dynamics on the widefield total internal reflection fluorescence imaging with high spatial and temporal resolution using the optical waveguide technique. The practical system was achieved after some sensitivity improvements in an optical waveguide imaging, which has succeeded in detecting very weak fluorescence signals reflecting single-cell secretion dynamics in spite of using a low magnification objective lens. Our methodology is more advantageous than other traditional microscopic technologies in real-time imaging of cell-to-cell communication through humoral factor secretion among actively migrating cells, like immune cells do in vivo.",

keywords = "Single cell, live cell imaging, optical waveguide, secretion",

author = "Yumiko Tanaka and Nobutake Suzuki and Kazuyo Mora and Jun Mizuno and Shuichi Shoji and Sotaro Uemura and Yoshitaka Shirasaki",

note = "Funding Information: This work was supported by JSPS KAKENHI on Innovative Areas (15H01366) and partly by JSPS KAKENHI (A) (16H02349) from the MEXT, Japan. Publisher Copyright: {\textcopyright} 2017 IEEE.; 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 ; Conference date: 18-06-2017 Through 22-06-2017",

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AU - Tanaka, Yumiko

AU - Suzuki, Nobutake

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AU - Uemura, Sotaro

AU - Shirasaki, Yoshitaka

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N2 - We report the novel methodology which enables us to analyze single-cell secretion dynamics on the widefield total internal reflection fluorescence imaging with high spatial and temporal resolution using the optical waveguide technique. The practical system was achieved after some sensitivity improvements in an optical waveguide imaging, which has succeeded in detecting very weak fluorescence signals reflecting single-cell secretion dynamics in spite of using a low magnification objective lens. Our methodology is more advantageous than other traditional microscopic technologies in real-time imaging of cell-to-cell communication through humoral factor secretion among actively migrating cells, like immune cells do in vivo.

AB - We report the novel methodology which enables us to analyze single-cell secretion dynamics on the widefield total internal reflection fluorescence imaging with high spatial and temporal resolution using the optical waveguide technique. The practical system was achieved after some sensitivity improvements in an optical waveguide imaging, which has succeeded in detecting very weak fluorescence signals reflecting single-cell secretion dynamics in spite of using a low magnification objective lens. Our methodology is more advantageous than other traditional microscopic technologies in real-time imaging of cell-to-cell communication through humoral factor secretion among actively migrating cells, like immune cells do in vivo.

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Widefield real-time single-cell secretion imaging with optical waveguide technique

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Keywords

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