Yeast formins Bni1 and Bnr1 utilize different modes of cortical interaction during the assembly of actin cables

Shawnna M. Buttery, Satoshi Yoshida, David Pellman

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The budding yeast formins Bni1 and Bnr1 control the assembly of actin cables. These formins exhibit distinct patterns of localization and polymerize two different populations of cables: Bni1 in the bud and Bnr1 in the mother cell. We generated a functional Bni1-3GFP that improved the visualization of Bni1 in vivo at endogenous levels. Bni1 exists as speckles in the cytoplasm, some of which colocalize on actin cables. These Bni1 speckles display linear, retrograde-directed movements. Loss of polymerized actin or specifically actin cables abolished retrograde movement, and resulted in depletion of Bni1 speckles from the cytoplasm, with enhanced targeting of Bni1 to the bud tip. Mutations that impair the actin assembly activity of Bni1 abolished the movement of Bni1 speckles, even when actin cables were present. In contrast, Bnr1-GFP or 3GFP-Bnr1 did not detectably associate with actin cables and was not observed as cytoplasmic speckles. Finally, fluorescence recovery after photobleaching demonstrated that Bni1 was very dynamic, exchanging between polarized sites and the cytoplasm, whereas Bnr1 was confined to the bud neck and did not exchange with a cytoplasmic pool. In summary, our results indicate that formine can have distinct modes of cortical interaction during actin cable assembly.

Original languageEnglish
Pages (from-to)1826-1838
Number of pages13
JournalMolecular Biology of the Cell
Volume18
Issue number5
DOIs
Publication statusPublished - 2007 May 1
Externally publishedYes

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Actins
Yeasts
Cytoplasm
Fluorescence Recovery After Photobleaching
Saccharomycetales
Stem Cells
Mutation
Population

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Yeast formins Bni1 and Bnr1 utilize different modes of cortical interaction during the assembly of actin cables. / Buttery, Shawnna M.; Yoshida, Satoshi; Pellman, David.

In: Molecular Biology of the Cell, Vol. 18, No. 5, 01.05.2007, p. 1826-1838.

Research output: Contribution to journalArticle

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