Ypk1 and Ypk2 kinases maintain Rho1 at the plasma membrane by flippase-dependent lipid remodeling after membrane stresses

Riko Hatakeyama, Keiko Kono, Satoshi Yoshida

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The plasma membrane (PM) is frequently challenged by mechanical stresses. In budding yeast, TORC2-Ypk1/Ypk2 kinase cascade plays a crucial role in PM stress responses by reorganizing the actin cytoskeleton via Rho1 GTPase. However, the molecular mechanism by which TORC2-Ypk1/Ypk2 regulates Rho1 is not well defined. Here, we found that Ypk1/Ypk2 maintain PM localization of Rho1 under PM stress via spatial reorganization of the lipids including phosphatidylserine. Genetic evidence suggests that this process is mediated by the Lem3-containing lipid flippase.We propose that lipid remodeling mediated by the TORC2-Ypk1/Ypk2-Lem3 axis is a backup mechanism for PM anchoring of Rho1 after PM stressinduced acute degradation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], which is responsible for Rho1 localization under normal conditions. Since all the signaling molecules studied here are conserved in higher eukaryotes, our findings might represent a general mechanism to cope with PM stress.

Original languageEnglish
Pages (from-to)1169-1178
Number of pages10
JournalJournal of Cell Science
Volume130
Issue number6
DOIs
Publication statusPublished - 2017 Jan 1
Externally publishedYes

Fingerprint

Phosphotransferases
Cell Membrane
Lipids
Membranes
Mechanical Stress
Saccharomycetales
GTP Phosphohydrolases
Phosphatidylserines
Phosphatidylinositols
Eukaryota
Actin Cytoskeleton
TOR complex 2

Keywords

  • Actin cytoskeleton
  • Akt
  • Flippase
  • Membrane stress
  • Phosphatidylserine
  • Rho

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ypk1 and Ypk2 kinases maintain Rho1 at the plasma membrane by flippase-dependent lipid remodeling after membrane stresses. / Hatakeyama, Riko; Kono, Keiko; Yoshida, Satoshi.

In: Journal of Cell Science, Vol. 130, No. 6, 01.01.2017, p. 1169-1178.

Research output: Contribution to journalArticle

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