TY - JOUR
T1 - Zealpeptaibolin, an 11-mer cytotoxic peptaibol group with 3 Aib-Pro motifs isolated from Trichoderma sp. RK10-F026
AU - Rawa, Mira Syahfriena Amir
AU - Nogawa, Toshihiko
AU - Okano, Akiko
AU - Futamura, Yushi
AU - Wahab, Habibah A.
AU - Osada, Hiroyuki
N1 - Funding Information:
Acknowledgements The authors thank Ms N Morita and Mr T Abu-kawa for the collection of a soil, Dr. JAV Lopez for the hydrophobicity calculation, and Ms H Aono, Ms E Sanada, Dr M Uchida, Dr R Uson-Lopez, Ms K Watanabe for the biological activity tests. This work was supported in part by JSPS KAKENHI (grant numbers JP17H06412 and JP20K05858).
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to the Japan Antibiotics Research Association.
PY - 2021/8
Y1 - 2021/8
N2 - Six new 11-mer peptaibols designed as zealpeptaibolins, A – F were isolated from the soil fungus, Trichoderma sp. RK10-F026, based on the principal component analysis of the MS data from five different culture compositions. Previously, 20-mer peptaibols from the same fungal strain were identified; 11-mer peptaibols in contrast were discovered from a different culture condition, signifying peptaibol production was culture condition-dependent. These peptaibols contained three Aib-Pro motifs in the sequence. The structures were established by NMR and HR-MS experiments including detailed MS/MS fragmentations. The absolute configurations were determined by Marfey’s analysis. Zealpeptaibolin F exhibited the strongest cytotoxicity toward K562 leukemia cells with an IC50 value of 0.91 µM.
AB - Six new 11-mer peptaibols designed as zealpeptaibolins, A – F were isolated from the soil fungus, Trichoderma sp. RK10-F026, based on the principal component analysis of the MS data from five different culture compositions. Previously, 20-mer peptaibols from the same fungal strain were identified; 11-mer peptaibols in contrast were discovered from a different culture condition, signifying peptaibol production was culture condition-dependent. These peptaibols contained three Aib-Pro motifs in the sequence. The structures were established by NMR and HR-MS experiments including detailed MS/MS fragmentations. The absolute configurations were determined by Marfey’s analysis. Zealpeptaibolin F exhibited the strongest cytotoxicity toward K562 leukemia cells with an IC50 value of 0.91 µM.
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U2 - 10.1038/s41429-021-00429-y
DO - 10.1038/s41429-021-00429-y
M3 - Article
C2 - 34163024
AN - SCOPUS:85108500559
SN - 0021-8820
VL - 74
SP - 485
EP - 495
JO - Journal of Antibiotics
JF - Journal of Antibiotics
IS - 8
ER -