Zealpeptaibolin, an 11-mer cytotoxic peptaibol group with 3 Aib-Pro motifs isolated from Trichoderma sp. RK10-F026

Mira Syahfriena Amir Rawa, Toshihiko Nogawa, Akiko Okano, Yushi Futamura, Habibah A. Wahab, Hiroyuki Osada*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Six new 11-mer peptaibols designed as zealpeptaibolins, A – F were isolated from the soil fungus, Trichoderma sp. RK10-F026, based on the principal component analysis of the MS data from five different culture compositions. Previously, 20-mer peptaibols from the same fungal strain were identified; 11-mer peptaibols in contrast were discovered from a different culture condition, signifying peptaibol production was culture condition-dependent. These peptaibols contained three Aib-Pro motifs in the sequence. The structures were established by NMR and HR-MS experiments including detailed MS/MS fragmentations. The absolute configurations were determined by Marfey’s analysis. Zealpeptaibolin F exhibited the strongest cytotoxicity toward K562 leukemia cells with an IC50 value of 0.91 µM.

Original languageEnglish
Pages (from-to)485-495
Number of pages11
JournalJournal of Antibiotics
Volume74
Issue number8
DOIs
Publication statusPublished - 2021 Aug
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

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