Abstract
MDPC-23 cells were incubated with ZnCl<inf>2</inf> and the levels of HO-1 protein, phosphorylated forms of mitogen-activated protein kinases (MAPKs), including extracellular signal-regulated protein kinase (ERK), c-Jun NH<inf>2</inf>-terminal kinase (JNK), and p38 were determined with western immunoblotting. The level of HO-1 mRNA was determined with RT-PCR analysis. After pretreatment with inhibitors of MAPKs, HO-1 protein level was determined in MDPC-23 cells exposed to ZnCl<inf>2</inf>. Following exposure to 500 μ M ZnCl<inf>2</inf>, the levels of both HO-1 mRNA and protein were markedly increased. The phosphorylated forms of MAPKs increased after ZnCl<inf>2</inf> exposure. Furthermore, the expression of HO-1 was markedly suppressed by treatment with the p38 inhibitor, SB203580, and mildly suppressed by the MAPK/ERK kinase inhibitor, U0126. However, treatment with the JNK inhibitor, SP600125, did not suppress ZnCl<inf>2</inf>-induced HO-1 expression. These results suggest that ZnCl<inf>2</inf> exposure induces HO-1 expression via multiple intracellular signalling pathways, including p38, ERK in this odontoblast-like cell lines.
| Original language | English |
|---|---|
| Pages (from-to) | O-215-O-216 |
| Journal | Transactions of Japanese Society for Medical and Biological Engineering |
| Volume | 52 |
| DOIs | |
| Publication status | Published - 2014 Aug 17 |
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Keywords
- HO-1
- Odontoblast-like cells
- Zinc chloride
ASJC Scopus subject areas
- Biomedical Engineering
Cite this
Zinc chloride exposure increases heme oxygenase-1 expression in odontoblast-like cells. / Karube, Hiroyo.
In: Transactions of Japanese Society for Medical and Biological Engineering, Vol. 52, 17.08.2014, p. O-215-O-216.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Zinc chloride exposure increases heme oxygenase-1 expression in odontoblast-like cells
AU - Karube, Hiroyo
PY - 2014/8/17
Y1 - 2014/8/17
N2 - MDPC-23 cells were incubated with ZnCl2 and the levels of HO-1 protein, phosphorylated forms of mitogen-activated protein kinases (MAPKs), including extracellular signal-regulated protein kinase (ERK), c-Jun NH2-terminal kinase (JNK), and p38 were determined with western immunoblotting. The level of HO-1 mRNA was determined with RT-PCR analysis. After pretreatment with inhibitors of MAPKs, HO-1 protein level was determined in MDPC-23 cells exposed to ZnCl2. Following exposure to 500 μ M ZnCl2, the levels of both HO-1 mRNA and protein were markedly increased. The phosphorylated forms of MAPKs increased after ZnCl2 exposure. Furthermore, the expression of HO-1 was markedly suppressed by treatment with the p38 inhibitor, SB203580, and mildly suppressed by the MAPK/ERK kinase inhibitor, U0126. However, treatment with the JNK inhibitor, SP600125, did not suppress ZnCl2-induced HO-1 expression. These results suggest that ZnCl2 exposure induces HO-1 expression via multiple intracellular signalling pathways, including p38, ERK in this odontoblast-like cell lines.
AB - MDPC-23 cells were incubated with ZnCl2 and the levels of HO-1 protein, phosphorylated forms of mitogen-activated protein kinases (MAPKs), including extracellular signal-regulated protein kinase (ERK), c-Jun NH2-terminal kinase (JNK), and p38 were determined with western immunoblotting. The level of HO-1 mRNA was determined with RT-PCR analysis. After pretreatment with inhibitors of MAPKs, HO-1 protein level was determined in MDPC-23 cells exposed to ZnCl2. Following exposure to 500 μ M ZnCl2, the levels of both HO-1 mRNA and protein were markedly increased. The phosphorylated forms of MAPKs increased after ZnCl2 exposure. Furthermore, the expression of HO-1 was markedly suppressed by treatment with the p38 inhibitor, SB203580, and mildly suppressed by the MAPK/ERK kinase inhibitor, U0126. However, treatment with the JNK inhibitor, SP600125, did not suppress ZnCl2-induced HO-1 expression. These results suggest that ZnCl2 exposure induces HO-1 expression via multiple intracellular signalling pathways, including p38, ERK in this odontoblast-like cell lines.
KW - HO-1
KW - Odontoblast-like cells
KW - Zinc chloride
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U2 - 10.11239/jsmbe.52.O-215
DO - 10.11239/jsmbe.52.O-215
M3 - Article
VL - 52
SP - O-215-O-216
JO - BME = Bio medical engineering / henshu, Nihon ME Gakkai
JF - BME = Bio medical engineering / henshu, Nihon ME Gakkai
SN - 1347-443X
ER -