Zirconia substrate with periodic surface microstructures enhances osteogenic differentiation of rat adipose-derived stem cells

Mayu Yasunaga*, Tomoko Watanabe, Gen Yano, Kazutoshi Murotomi, Miki Hiramatsu, Motohiro Hirose, Masayuki Kakehata, Hidehiko Yashiro, Atsushi Yamazaki, Atsuo Ito

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Zirconia substrates with periodic surface microstructures formed by irradiation with a femtosecond laser previously enhanced osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), associated with a reduction in the cell attachment area. Adipose-derived mesenchymal stem cells (ADSCs) are clinically less difficult in harvesting the cells than BMSCs. In this study, we investigated the effects of zirconia substrates with the periodic surface microstructures on osteogenic differentiation of rat ADSCs. Zirconia substrates with the periodic surface microstructures enhanced not adipogenic differentiation but osteogenic differentiation of rat ADSCs, associated with a reduction in the cell attachment area. Therefore, zirconia substrates with surface periodic microstructures formed by irradiation with a femtosecond laser could be useful in bone tissue engineering using ADSCs when BMSCs are unavailable.

Original languageEnglish
Article number133544
JournalMaterials Letters
Volume332
DOIs
Publication statusAccepted/In press - 2022

Keywords

  • Adipose-derived mesenchymal stem cells
  • Cell attachment area
  • Femtosecond laser irradiation
  • Osteogenic differentiation
  • Zirconia substrates

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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