βIV-spectrin forms a diffusion barrier against L1CAM at the axon initial segment

Kazunari Nishimura, Hiroki Akiyama, Masayuki Komada, Hiroyuki Kamiguchi*

*この研究の対応する著者

研究成果査読

21 被引用数 (Scopus)

抄録

Axonal and somatodendritic plasma membranes of polarized neurons express distinct sets of functional molecules. It is known that the neuronal polarity can be maintained by a barrier that impedes diffusional mixing of membrane components between the two domains. Using βIV-spectrin knockout mice, we demonstrate the involvement of this cytoskeletal protein in the formation of a barrier that selectively blocks lateral mobility of L1 cell adhesion molecule (L1CAM) at the axon initial segment of hippocampal neurons. We also show that the βIV-spectrin-based barrier is required for the axon-specific distribution of L1CAM both in vitro and in vivo. The barrier activity against L1CAM may depend on direct interactions of L1CAM with ankyrinG, a protein binding to βIV-spectrin, rather than on steric hindrance by other transmembrane proteins clustered at the axon initial segment. Our results highlight the role of βIV-spectrin and ankyrinG as critical components of a selective barrier against L1CAM.

本文言語English
ページ(範囲)422-430
ページ数9
ジャーナルMolecular and Cellular Neuroscience
34
3
DOI
出版ステータスPublished - 2007 3
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 細胞および分子神経科学
  • 発達神経科学

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