The production of γ-linolenic acid from algae in fresh and marine water was investigated. When Spirulina platensis was left in the dark condition, it contained about one and a half times γ-linolenic acid compared with conventional Spirulina platensis. Marine microalga, Chlorella sp. NKG 042401 contained about 10% of γ-linolenic acid. The highest γ-linolenic acid content was obtained when this alga was cultured under the radiation of around 100 μEinstein/m2/s.
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