16s rRNA-targeted identification of cyanobacterial genera using oligonucleotide-probes immobilized on bacterial magnetic particles

Tadashi Matsunaga, Haruko Takeyama, Hideki Nakayama

研究成果: Article査読

7 被引用数 (Scopus)

抄録

16S rRNA-targeted identification of cyanobacterial genera, Anabaena, Microcystis, Nostoc, Oscillatoria, Synechococcus was developed using bacterial magnetic particles (BMPs). 16S rRNA-targeted capture probes designed from the genus specific region of the 16S rRNA sequence were immobilized on BMPs. Identification of cyanobacteria was performed by a sandwich hybridization using the capture probes - BMP conjugates and a digoxigenin (DIG)-labeled detector probe complementary to the highly conserved 16S rRNA sequence for cyanobacteria. The luminescence intensity of the probe/target-BMP hybrids was measured after reaction with alkaline phosphatase conjugated anti-DIG antibody. Five species of cyanobacteria from five different genera were successfully discriminated using this magnetic capture system.

本文言語English
ページ(範囲)389-394
ページ数6
ジャーナルJournal of Applied Phycology
13
4
DOI
出版ステータスPublished - 2001

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

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