2,2,2-tribromoethanol phase-shifts the circadian rhythm of the liver clock in Per2::Luciferase knockin mice: Lack of dependence on anesthetic activity

Yuji Kubo, Yu Tahara, Akiko Hirao, Shigenobu Shibata*

*この研究の対応する著者

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Comprehensive gene expression profiling in mice in response to the inhalation of sevoflurane has revealed that circadian clock gene expression is affected strongly in the liver, heart, lung, and kidney, in this order, but moderately in the spleen and slightly in the brain. Therefore, we examined whether the administration of general anesthetics at different times of the day induces phase shifts of the liver clock in Per2::Luciferase knockin mice. One to 4 days of intraperitoneal injection of 2,2,2-tribromoethanol (240 mg/kg, anesthetic time 60 min) or 2,2,2-trichloroethanol (240 mg/kg, 60 min), common anesthetics in veterinary surgery, caused phase delays when injected during the daytime and phase advances when injected during the nighttime. Inhalation administration of isoflurane for 30 or 60 min during the daytime did not induce a phase delay. Injection of propofol (300 mg/kg, 17 min) during the daytime induced an insignificant phase delay of the Per2 bioluminescence rhythm. Injection of 2,2,2-tribromoethanol did not induce a phase shift in the suprachiasmatic nucleus, the main oscillator, or in behavioral locomotor rhythms, suggesting that 2,2,2-tribromoethanol induced phase shifts of the liver clock independent of the main suprachiasmatic clock. The expression of clock genes, such as Bmal1 and Clock, in mouse liver was decreased strongly 1 and 4 h after a single injection of 2,2,2- tribromoethanol. These results demonstrate that 2,2,2-tribromoethanol or 2,2,2-trichloroethanol produce phase shifts of the peripheral clock, independent of anesthetic activity. These anesthetics may cause circadian rhythm disorders in peripheral organs when administered as general anesthetics several times during the day.

本文言語English
ページ(範囲)698-705
ページ数8
ジャーナルJournal of Pharmacology and Experimental Therapeutics
340
3
DOI
出版ステータスPublished - 2012 3

ASJC Scopus subject areas

  • 分子医療
  • 薬理学

フィンガープリント

「2,2,2-tribromoethanol phase-shifts the circadian rhythm of the liver clock in Per2::Luciferase knockin mice: Lack of dependence on anesthetic activity」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル