A bacterial continuous culture system based on a microfluidic droplet open reactor

Manami Ito, Haruka Sugiura, Shotaro Ayukawa, Daisuke Kiga, Masahiro Takinoue

研究成果: Article

1 引用 (Scopus)

抜粋

Recently, micrometer-sized bacterial culture systems have attracted attention as useful tools for synthetic biology studies. Here, we present the development of a bacterial continuous culture system based on a microdroplet open reactor consisting of two types of water-in-oil microdroplets with diameters of several hundred micrometers. A continuous culture was realized the through supply of nutrient substrates and the removal of waste and excess bacterial cells based on repeated fusion and fission of droplets. The growth dynamics was controlled by the interval of fusion. We constructed a microfluidic system and quantitatively assessed the dynamics of the bacterial growth using a mathematical model. This system will facilitate the study of synthetic biology and metabolic engineering in the future.

元の言語English
ページ(範囲)61-66
ページ数6
ジャーナルAnalytical Sciences
32
発行部数1
DOI
出版物ステータスPublished - 2016

ASJC Scopus subject areas

  • Analytical Chemistry

フィンガープリント A bacterial continuous culture system based on a microfluidic droplet open reactor' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用