A catalytic immuno-reactor for the amperometric determination of human serum albumin

Isao Karube, Tadashi Matsunaga, Takeru Satoh, Shuichi Suzuki

研究成果: Article査読

6 被引用数 (Scopus)

抄録

A catalytic immuno-reactor for the determination of human serum albumin (HSA) was constructed by using immobilized antibody and an amperometric detector. A sandwich assay with hemin-labeled antibody to catalyze the decomposition of hydrogen peroxide was used, the catalytic activity of the hemin-antibody conjugate being determined by measuring the decrease in hydrogen peroxide concentration. The reaction of hemin-labeled antibody with antigen was complete within 30 min and the current decrease was correlated with the HSA concentration. The relative standard deviation was about 9% at an HSA concentration of 1 mg ml-1.

本文言語English
ページ(範囲)283-287
ページ数5
ジャーナルAnalytica Chimica Acta
156
C
DOI
出版ステータスPublished - 1984
外部発表はい

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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