A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules

Kento Yamagishi; Kazuaki Sawaki; Atsushi Murata; Shinji Takeoka

doi:10.1039/c4cc09947h

A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules

Kento Yamagishi, Kazuaki Sawaki, Atsushi Murata, Shinji Takeoka

ナノ・ライフ創新研究機構

研究成果: Article › 査読

8 被引用数 (Scopus)

抄録

We synthesized a novel cyclooctyne-based clickable fluorescent probe with versatile properties such as high cell-membrane permeability and free diffusibility in the cell. Our probe "FC-DBCO" was conjugated to an azide-modified mannose via a Cu-free click reaction in living HeLa cells and displayed intracellular specific fluorescence imaging with low background signals.

本文言語	English
ページ（範囲）	7879-7882
ページ数	4
ジャーナル	Chemical Communications
巻	51
号	37
DOI	https://doi.org/10.1039/c4cc09947h
出版ステータス	Published - 2015 5 7

ASJC Scopus subject areas

Chemistry(all)
Catalysis
Ceramics and Composites
Electronic, Optical and Magnetic Materials
Surfaces, Coatings and Films
Materials Chemistry
Metals and Alloys

文献へのアクセス

10.1039/c4cc09947h

他のファイルとリンク

フィンガープリント「A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

@article{18a4cf1b92074416be052e87226798cf,

title = "A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules",

abstract = "We synthesized a novel cyclooctyne-based clickable fluorescent probe with versatile properties such as high cell-membrane permeability and free diffusibility in the cell. Our probe {"}FC-DBCO{"} was conjugated to an azide-modified mannose via a Cu-free click reaction in living HeLa cells and displayed intracellular specific fluorescence imaging with low background signals.",

author = "Kento Yamagishi and Kazuaki Sawaki and Atsushi Murata and Shinji Takeoka",

year = "2015",

month = may,

day = "7",

doi = "10.1039/c4cc09947h",

language = "English",

volume = "51",

pages = "7879--7882",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "37",

}

TY - JOUR

T1 - A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules

AU - Yamagishi, Kento

AU - Sawaki, Kazuaki

AU - Murata, Atsushi

AU - Takeoka, Shinji

PY - 2015/5/7

Y1 - 2015/5/7

N2 - We synthesized a novel cyclooctyne-based clickable fluorescent probe with versatile properties such as high cell-membrane permeability and free diffusibility in the cell. Our probe "FC-DBCO" was conjugated to an azide-modified mannose via a Cu-free click reaction in living HeLa cells and displayed intracellular specific fluorescence imaging with low background signals.

AB - We synthesized a novel cyclooctyne-based clickable fluorescent probe with versatile properties such as high cell-membrane permeability and free diffusibility in the cell. Our probe "FC-DBCO" was conjugated to an azide-modified mannose via a Cu-free click reaction in living HeLa cells and displayed intracellular specific fluorescence imaging with low background signals.

UR - http://www.scopus.com/inward/record.url?scp=84928522220&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928522220&partnerID=8YFLogxK

U2 - 10.1039/c4cc09947h

DO - 10.1039/c4cc09947h

M3 - Article

C2 - 25854695

AN - SCOPUS:84928522220

VL - 51

SP - 7879

EP - 7882

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 37

ER -