A cytotoxic ribonuclease targeting specific transfer RNA anticodons

T. Ogawa, K. Tomita, T. Ueda, K. Watanabe, T. Uozumi, H. Masaki*

*この研究の対応する著者

研究成果: Article査読

137 被引用数 (Scopus)

抄録

The carboxyl-terminal domain of colicin E5 was shown to inhibit protein synthesis of Escherichia coli. Its target, as revealed through in vivo and in vitro experiments, was not ribosomes as in the case of E3, but the transfer RNAs (tRNAs) for Tyr, His, Ash, and Asp, which contain a modified base, queuine, at the wobble position of each anticodon. The E5 carboxyl-terminal domain hydrolyzed these tRNAs just on the 3' side of this nucleotide. Tight correlation was observed between the toxicity of E5 and the cleavage of intracellular tRNAs of this group, implying that these tRNAs are the primary targets of colicin E5.

本文言語English
ページ(範囲)2097-2100
ページ数4
ジャーナルScience
283
5410
DOI
出版ステータスPublished - 1999 3 26
外部発表はい

ASJC Scopus subject areas

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