A DIGE proteomic analysis for low-intensity exercise-trained rat skeletal muscle

Wataru Yamaguchi*, Eri Takai, Mitsuru Higuchi, Izumi Tabata


研究成果: Article査読

2 被引用数 (Scopus)


Background: Exercise training induces various adaptations in skeletal muscles. However, the mechanisms remain unclear. Purpose: Therefore, we conducted 2D-DIGE proteomic analysis, which has not yet been used for elucidating adaptations of skeletal muscle after low-intensity exercise training (LIT). Methods: For five days, rats performed LIT, which consisted of two 3-h swimming exercise with45-m rest between the exercise bouts. 2D-DIGE analysis was conducted on epitrochlearis muscles excised eighteen hours after the final training exercise. Results: Proteomic profiling revealed that, out of 681 detected and matched spots, 22 proteins exhibited changed expression by LIT compared with sedentary rats. All proteins were identified by MALDI-TOF/MS. Conclusion: The proteomic 2D-DIGE analysis following LIT identified expressions of skeletal muscle proteins, includingATPsyna, UQCRC1, dihydrolipoamide dehydrogenase, dihydrolipoamide acetyltransferase, that were not previously reported to change their expressions after exercise-training.

ジャーナルJapanese Journal of Physical Fitness and Sports Medicine
出版ステータスPublished - 2011

ASJC Scopus subject areas

  • 整形外科およびスポーツ医学
  • 理学療法、スポーツ療法とリハビリテーション


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