A histone methyltransferase ESET is critical for T cell development

Shoichi Takikita, Ryunosuke Muro, Toshiyuki Takai, Takeshi Otsubo, Yuki I. Kawamura, Taeko Dohi, Hiroyo Oda, Masayuki Kitajima, Kenshiro Oshima, Masahira Hattori, Takaho A. Endo, Tetsuro Toyoda, John Weis, Yoichi Shinkai, Harumi Suzuki

    研究成果: Article

    8 引用 (Scopus)

    抜粋

    ESET/SETDB1, one of the major histone methyltransferases, catalyzes histone 3 lysine 9 (H3K9) trimethylation. ESET is critical for suppressing expression of retroviral elements in embryonic stem cells; however, its role in the immune system is not known.We found that thymocyte-specific deletion of ESET caused impaired T cell development, with CD8 lineage cells being most severely affected. Increased apoptosis of CD8 single-positive cells was observed, and TCR-induced ERK activation was severely inhibited in ESET2/2 thymocytes. Genome-wide comprehensive analysis of mRNA expression and H3K9 trimethylation revealed that ESET regulates expression of numerous genes in thymocytes. Among them, FcgRIIB, whose signaling can inhibit ERK activation, was strongly and ectopically expressed in ESET2/2 thymocytes. Indeed, genetic depletion of FcgRIIB in ESET2/2 thymocytes rescued impaired ERK activation and partially restored defective positive selection in ESET2/2 mice. Therefore, impaired T cell development in ESET2/2 mice is partly due to the aberrant expression of FcgRIIB. Collectively, to our knowledge, we identify ESET as the first trimethylated H3K9 histone methyltransferase playing a crucial role in T cell development.

    元の言語English
    ページ(範囲)2269-2279
    ページ数11
    ジャーナルJournal of Immunology
    197
    発行部数6
    DOI
    出版物ステータスPublished - 2016 9 15

      フィンガープリント

    ASJC Scopus subject areas

    • Immunology

    これを引用

    Takikita, S., Muro, R., Takai, T., Otsubo, T., Kawamura, Y. I., Dohi, T., Oda, H., Kitajima, M., Oshima, K., Hattori, M., Endo, T. A., Toyoda, T., Weis, J., Shinkai, Y., & Suzuki, H. (2016). A histone methyltransferase ESET is critical for T cell development. Journal of Immunology, 197(6), 2269-2279. https://doi.org/10.4049/jimmunol.1502486