A hypothesis about myosin catalysis

Hirofumi Onishi*, Takashi Ohki, Naoki Mozhizuki, Manuel F. Morales

*この研究の対応する著者

研究成果: Article査読

抄録

When ATP binds to the active site of myosin heads, Switch II undergoes a large conformational change and the cleft surrounding the bound γ-phosphate closes. In the closed state, Glu470 in Switch II comes together with Arg247 in Switch I to form a salt-bridge. Here, the functional significance of the two bridging residues was tested by using site-directed mutagenesis. We conclude from such tests that (a) the attractive force between Arg247 and the γ-phosphate of ATP moves the cleft to close, and (b) during hydrolysis, Glu470 is intimately involved in positioning the lytic water for the attack on the γ-phosphorus. We also speculate on how the salt-bridge between Arg247 and Glu470 is related to hydrolysis.

本文言語English
ページ(範囲)175-181
ページ数7
ジャーナルAdvances in Experimental Medicine and Biology
538
出版ステータスPublished - 2004
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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