A 'macro' image analysis of fura-2 fluorescence to visualize the distribution of functional glutamate receptor subtypes in hippocampal slices

Yoshihisa Kudo, Takeshi Nakamura, Etsuro Ito

    研究成果: Article

    35 引用 (Scopus)

    抄録

    The distribution of functional glutamate receptor subtypes was visualized as regional changes in intracellular Ca2+ concentration induced by administration of a specific agonist for each subtype. An image of the fura-2 fluorescence from a whole hippocampal slice was directly excited at 340 or 380 nm through an optical fibre and observed via a low-magnification objective lens (× 4). A heterogeneous distribution of N-methyl-d-aspartate (NMDA) and non-NMDA subtypes was demonstrated by the ratio of fluorescence intensities before and after administration of each agonist. This 'macro' image analysis can reveal distributions of multiple functional receptors in a living brain slice.

    元の言語English
    ページ(範囲)412-420
    ページ数9
    ジャーナルNeuroscience Research
    12
    発行部数3
    DOI
    出版物ステータスPublished - 1991

    Fingerprint

    Fura-2
    Glutamate Receptors
    Aspartic Acid
    Fluorescence
    Optical Fibers
    Lenses
    Brain

    ASJC Scopus subject areas

    • Neuroscience(all)

    これを引用

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    abstract = "The distribution of functional glutamate receptor subtypes was visualized as regional changes in intracellular Ca2+ concentration induced by administration of a specific agonist for each subtype. An image of the fura-2 fluorescence from a whole hippocampal slice was directly excited at 340 or 380 nm through an optical fibre and observed via a low-magnification objective lens (× 4). A heterogeneous distribution of N-methyl-d-aspartate (NMDA) and non-NMDA subtypes was demonstrated by the ratio of fluorescence intensities before and after administration of each agonist. This 'macro' image analysis can reveal distributions of multiple functional receptors in a living brain slice.",
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