A 'macro' image analysis of fura-2 fluorescence to visualize the distribution of functional glutamate receptor subtypes in hippocampal slices

Yoshihisa Kudo, Takeshi Nakamura, Etsuro Ito

研究成果: Article

35 引用 (Scopus)

抜粋

The distribution of functional glutamate receptor subtypes was visualized as regional changes in intracellular Ca2+ concentration induced by administration of a specific agonist for each subtype. An image of the fura-2 fluorescence from a whole hippocampal slice was directly excited at 340 or 380 nm through an optical fibre and observed via a low-magnification objective lens (× 4). A heterogeneous distribution of N-methyl-d-aspartate (NMDA) and non-NMDA subtypes was demonstrated by the ratio of fluorescence intensities before and after administration of each agonist. This 'macro' image analysis can reveal distributions of multiple functional receptors in a living brain slice.

元の言語English
ページ(範囲)412-420
ページ数9
ジャーナルNeuroscience Research
12
発行部数3
DOI
出版物ステータスPublished - 1991 11

ASJC Scopus subject areas

  • Neuroscience(all)

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