@article{447302bea1dc4bc9becf3cba8ab09691,
title = "A 'macro' image analysis of fura-2 fluorescence to visualize the distribution of functional glutamate receptor subtypes in hippocampal slices",
abstract = "The distribution of functional glutamate receptor subtypes was visualized as regional changes in intracellular Ca2+ concentration induced by administration of a specific agonist for each subtype. An image of the fura-2 fluorescence from a whole hippocampal slice was directly excited at 340 or 380 nm through an optical fibre and observed via a low-magnification objective lens (× 4). A heterogeneous distribution of N-methyl-d-aspartate (NMDA) and non-NMDA subtypes was demonstrated by the ratio of fluorescence intensities before and after administration of each agonist. This 'macro' image analysis can reveal distributions of multiple functional receptors in a living brain slice.",
keywords = "AMPA, Fura-2, Glutamate receptor subtypes, Hippocampal slice, Image analysis, Ionotropic receptor, Kainate, Metabotropic receptor, N-Methyl-d-aspartate (NMDA), t-ACPD",
author = "Yoshihisa Kudo and Takeshi Nakamura and Etsuro Ito",
note = "Funding Information: Part of this work was supported by a Grant-in-Aid for Scientific Research on Priority Areas of 'Impulse Signalling' (No. 01659002) from the Japanese Ministry of Education, Science and Culture. Valuable discussion with and advice from Dr.A. Ogura and Dr.Y. Ishida (Mitsubishi Kasei Institute of Life Sciences), and technical assistance by the staff of Hamamatsu Photonics Co. and by Ms. K. Akita are gratefully acknowledged.",
year = "1991",
month = nov,
doi = "10.1016/0168-0102(91)90072-7",
language = "English",
volume = "12",
pages = "412--420",
journal = "Neuroscience Research",
issn = "0168-0102",
publisher = "Elsevier Ireland Ltd",
number = "3",
}