A method for evaluating nucleosome stability with a protein-binding fluorescent dye

Hiroyuki Taguchi, Naoki Horikoshi, Yasuhiro Arimura, Hitoshi Kurumizaka*

*この研究の対応する著者

研究成果: Article査読

48 被引用数 (Scopus)

抄録

Nucleosomes are extremely stable histone. -DNA complexes that form the building blocks of chromatin, which accommodates genomic DNA within the nucleus. The dynamic properties of chromatin play essential roles in regulating genomic DNA functions, such as DNA replication, recombination, repair, and transcription. Histones are the protein components of nucleosomes, and their diverse modifications and variants increase the versatility of nucleosome structures and their dynamics in chromatin. Therefore, a technique to evaluate the physical properties of nucleosomes would facilitate functional studies of the various nucleosomes. In this report, we describe a convenient assay for evaluating the thermal stability of nucleosomes in vitro.

本文言語English
ページ(範囲)119-126
ページ数8
ジャーナルMethods
70
2-3
DOI
出版ステータスPublished - 2014

ASJC Scopus subject areas

  • 分子生物学
  • 生化学、遺伝学、分子生物学(全般)

フィンガープリント

「A method for evaluating nucleosome stability with a protein-binding fluorescent dye」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル