A murine Thy-1.2 reporter vector containing a SV40 origin for rapid cloning and analysis of eukaryotic promoters

Yuzo Kadokawa, Takahiro Kusakabe, Yusuke Kamachi, Ken ichi Isobe, Hisato Kondoh, Takashi Ohyama

研究成果: Article査読

抄録

A new vector, pATO, was constructed for rapid cloning and analysis of eukaryotic promoters. When a recombinant pATO, carrying a promoter sequence in its multiple cloning site, was introduced into COS cells, Thy-1.2 protein was produced on the cell surface, and was easily identified by an fluorescein-conjugated anti-Thy-1.2 antibody. The intensity of the fluorescence reflected the strength of the inserted promoter. Since pATO could replicate efficiently in COS cells, the recombinant plasmids recovered from a single COS cell were sufficient to transform Escherichia coli cells. This plasmid is applicable for the rapid and labor saving cloning of promoter elements.

本文言語English
ページ(範囲)277-278
ページ数2
ジャーナルGene
153
2
DOI
出版ステータスPublished - 1995 2 14
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

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