A new method for identifying the amino acid attached to a particular RNA in the cell

Tsutomu Suzuki, Takuya Ueda, Kimitsuna Watanabe*

*この研究の対応する著者

研究成果: Article査読

15 被引用数 (Scopus)

抄録

To investigate the function of tRNAs or any other aminoacylable RNAs in vivo, it is important to be able to estimate the amounts and species of aminoacylated RNAs in living cells. We have developed a method of analyzing amino acids attached to particular tRNAs obtained from cells. After the ester bond between the amino acid and the 3'-adenosine moiety of a specific aminoacyl-tRNA is stabilized by acetylation of the amino acid with [14C]acetic anhydride, the aminoacyl-tRNA can be fished out with a solid-phase-attached DNA probe. The 14C-labeled acetylamino acid is then released from the thus purified acetyl-aminoacyl-tRNAs by alkaline treatment and detected by TLC analysis.

本文言語English
ページ(範囲)195-198
ページ数4
ジャーナルFEBS Letters
381
3
DOI
出版ステータスPublished - 1996 3月 4
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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