A New Protein Conformation Indicator Based on Biarsenical Fluorescein with an Extended Benzoic Acid Moiety

Jun Nakanishi, Mizuo Maeda, Yoshio Umezawa

研究成果: Article査読

22 被引用数 (Scopus)

抄録

We demonstrate herein a new protein conformation indicator based on biarsenical fluorescein with an extended benzoic acid moiety. The present indicator is reactive to a genetically introduced tetracysteine motif (Cys-Cys-Xaa-Xaa-Cys-Cys, where Xaa is a noncysteine amino acid) of proteins. Compared to the original biarsenical fluorescein (FlAsH) and the biarsenical Nile red analogue (BArNile), the present indicator exhibited larger fluorescence intensity changes in response to Ca 2+ -induced conformational rearrangements of calmodulin. A calculation of the highest occupied molecular orbital (HOMO) level of the benzoic acid moiety of the indicator molecule supports possible involvement of a photoinduced electron transfer (PET) process. These results indicate that the present indicator is useful for sensitive detection of protein conformational changes.

本文言語English
ページ(範囲)273-278
ページ数6
ジャーナルAnalytical Sciences
20
2
DOI
出版ステータスPublished - 2004 2
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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