A new relaxin-like gonad-stimulating peptide identified in the starfish Asterias amurensis

Masatoshi Mita, Misaki Daiya, Shogo Haraguchi, Kazuyoshi Tsutsui, Yoshitaka Nagahama

    研究成果: Article査読

    20 被引用数 (Scopus)

    抄録

    Relaxin-like gonad-stimulating peptide (RGP) of starfish Asterina pectinifera was the first invertebrate gonadotropin to have its chemical structure identified. However, it is unclear whether gonadotropic hormones in other species starfish are relaxin-like peptides. Thus, this study tried to identify the molecular structure of gonadotropic hormone in Asterias amurensis. As a result, we identified A. amurensis gonadotropic hormone as the RGP (AamRGP). The DNA sequence encoding AamRGP consisted of 330 base pairs with an open reading frame encoding a peptide of 109 amino acids (aa), including a signal peptide (26 aa), B-chain (20 aa), C-peptide (38 aa) and A-chain (25 aa). Comparing with A. pectinifera RGP (ApeRGP), the amino acid identity levels between AmaRGP and ApeRGP were 58% for the A-chain and 73% for the B-chain. Furthermore, chemical synthetic AamRGP induced gamete spawning and oocyte maturation in ovarian fragments of A. amurensis. In contrast, the ovary of A. pectinifera failed to respond to the AamRGP. This suggested that AamRGP is a new relaxin-like peptide.

    本文言語English
    ページ(範囲)144-149
    ページ数6
    ジャーナルGeneral and Comparative Endocrinology
    222
    DOI
    出版ステータスPublished - 2015 10 1

    ASJC Scopus subject areas

    • 内分泌学

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