A new simple method to introduce marker-free deletions in the Bacillus subtilis genome

Takuya Morimoto, Katsutoshi Ara, Katsuya Ozaki, Naotake Ogasawara

研究成果: Article査読

33 被引用数 (Scopus)

抄録

A genetic tool to introduce marker-free deletions is essential for multiple manipulations of genomes. We report a simple and efficient method to create marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counter-selection tool. Our method will be applicable to any bacterium in which introduction of the mazF cassette into the genome by double crossover homologous recombination is possible.

本文言語English
ページ(範囲)315-318
ページ数4
ジャーナルGenes and Genetic Systems
84
4
DOI
出版ステータスPublished - 2009
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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