A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer

Hideyuki Terazono, Hyonchol Kim, Masahito Hayashi, Akihiro Hattori, Fumimasa Nomura, Tomoyuki Kaneko, Kenji Yasuda

研究成果: Article

4 引用 (Scopus)

抜粋

A non-destructive method of collecting cultured cells after identifying their in situ functional characteristics is proposed. In this method, cells are cultivated on an alginate layer in a culture dish and released by spot application of a calcium chelate buffer that locally melts the alginate layer and enables the collection of cultured cells at the single-cell level. Primary hippocampal neurons, beating human embryonic stem (hES) cell-derived cardiomyocytes, and beating hES cell-derived cardiomyocyte clusters cultivated on an alginate layer were successfully released and collected with a micropipette. The collected cells were recultured while maintaining their physiological function, including beating, and elongated neurites. These results suggest that the proposed method may eventually facilitate the transplantation of ES- or iPS-derived cardiomyocytes and neurons differentiated in culture.

元の言語English
記事番号e42485
ジャーナルPloS one
7
発行部数8
DOI
出版物ステータスPublished - 2012 8 3
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

フィンガープリント A non-destructive culturing and cell sorting method for cardiomyocytes and neurons using a double alginate layer' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用