A novel approach to Au nanoparticle-based identification of DNA nanoarrays

Guo Jun Zhang*, Takashi Tanii, Yuzo Kanari, Chiaki Yasumuro, Takashi Matsukawa, Meishoku Masahara, Iwao Ohdomari

*この研究の対応する著者

研究成果: Article査読

2 被引用数 (Scopus)

抄録

The combination of electron beam lithography and gold nanoparticle-based detection method is subject to a novel high-resolution approach to detecting DNA nanoarrays. In this work, gold nanoparticle-based detection of DNA hybridization on nanostructured arrays is presented. The nanostructured arrays were created by electron beam lithography of a self-assembled monolayer. Amine groups, which are active moieties and are used for attachment of DNA, were introduced to the nanostructures, and the amine-modified structures were characterized by scanning Maxwell-stress microscopy (SMM) for seeing the modification process. The DNA probe covalently immobilized within the nanostructures was hybridized with a biotinylated target DNA. Streptavidin-gold conjugate was then bound to the biotin, thereby assembling inside the nanostructured arrays. The sequence-specific hybridization was imaged by atomic force microscopy (AFM). On the other hand, the activity of the DNA molecules within the nanostructured arrays was verified by fluorescence microscopy using streptavidin-Cy 5 conjugate instead of streptavidin-gold nanoparticles conjugate. On the basis of fluorescent detection, an alternative method has been developed for detection of DNA nanostructures, which will benefit the development of DNA chips.

本文言語English
ページ(範囲)4773-4778
ページ数6
ジャーナルFrontiers in Bioscience
12
12
DOI
出版ステータスPublished - 2007 5 1

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 免疫学および微生物学(全般)

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