A novel avian hypothalamic peptide inhibiting gonadotropin release

Kazuyoshi Tsutsui*, Etsuko Saigoh, Kazuyoshi Ukena, Hitoshi Teranishi, Yuko Fujisawa, Motoshi Kikuchi, Susumu Ishii, Peter J. Sharp

*この研究の対応する著者

研究成果: Article査読

619 被引用数 (Scopus)

抄録

The neuropeptide control of gonadotropin secretion at the level of the anterior pituitary gland is primarily through the stimulatory action of the hypothalamic decapeptide, gonadotropin-releasing hormone (GnRH), which was originally isolated from mammals and subsequently from nonmammals. To date, however, an inhibitory peptide of gonadotropin release is unknown in vertebrates. Here we show, in a bird, that the hypothalamus also contains a novel peptide which inhibits gonadotropin release. Acetic acid extracts of quail brains were passed through C-18 reversed-phase cartridges, and then the retained material was subjected to the reversed-phase and cation-exchange high-performance liquid chromatography (HPLC). The peptide was isolated from avian brain and shown to have the sequence Ser-IIe-Lys-Pro-Ser-Ala-Tyr-Leu-Pro-Leu-Arg-Phe-NH2. Cell bodies and terminals containing this peptide were localized immunohistochemically in the paraventricular nucleus and median eminence, respectively. This peptide inhibited, in a dose-related way, gonadotropin release from cultured quail anterior pituitaries. This is the first hypothalamic peptide inhibiting gonadotropin release reported in a vertebrate. We therefore term it gonadotropin-inhibitory hormone (GnIH). (C) 2000 Academic Press.

本文言語English
ページ(範囲)661-667
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
275
2
DOI
出版ステータスPublished - 2000 8 28
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生物理学
  • 分子生物学

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