A novel avian hypothalamic peptide inhibiting gonadotropin release

Kazuyoshi Tsutsui, Etsuko Saigoh, Kazuyoshi Ukena, Hitoshi Teranishi, Yuko Fujisawa, Motoshi Kikuchi, Susumu Ishii, Peter J. Sharp

研究成果: Article

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The neuropeptide control of gonadotropin secretion at the level of the anterior pituitary gland is primarily through the stimulatory action of the hypothalamic decapeptide, gonadotropin-releasing hormone (GnRH), which was originally isolated from mammals and subsequently from nonmammals. To date, however, an inhibitory peptide of gonadotropin release is unknown in vertebrates. Here we show, in a bird, that the hypothalamus also contains a novel peptide which inhibits gonadotropin release. Acetic acid extracts of quail brains were passed through C-18 reversed-phase cartridges, and then the retained material was subjected to the reversed-phase and cation-exchange high-performance liquid chromatography (HPLC). The peptide was isolated from avian brain and shown to have the sequence Ser-IIe-Lys-Pro-Ser-Ala-Tyr-Leu-Pro-Leu-Arg-Phe-NH2. Cell bodies and terminals containing this peptide were localized immunohistochemically in the paraventricular nucleus and median eminence, respectively. This peptide inhibited, in a dose-related way, gonadotropin release from cultured quail anterior pituitaries. This is the first hypothalamic peptide inhibiting gonadotropin release reported in a vertebrate. We therefore term it gonadotropin-inhibitory hormone (GnIH). (C) 2000 Academic Press.

元の言語English
ページ(範囲)661-667
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
275
発行部数2
DOI
出版物ステータスPublished - 2000 8 28
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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    Tsutsui, K., Saigoh, E., Ukena, K., Teranishi, H., Fujisawa, Y., Kikuchi, M., Ishii, S., & Sharp, P. J. (2000). A novel avian hypothalamic peptide inhibiting gonadotropin release. Biochemical and Biophysical Research Communications, 275(2), 661-667. https://doi.org/10.1006/bbrc.2000.3350