A novel complete reconstitution system for membrane integration of the simplest membrane protein

Ken ichi Nishiyama*, Masahide Maeda, Masato Abe, Takashi Kanamori, Keiko Shimamoto, Shoichi Kusumoto, Takuya Ueda, Hajime Tokuda

*この研究の対応する著者

研究成果: Article査読

32 被引用数 (Scopus)

抄録

A complete reconstitution system for membrane integration of the simplest protein was developed by means of defined factors. A mutant version of Pf3 coat protein, 3L-Pf3 coat, requires neither signal recognition particle/Sec factors nor a membrane potential for its integration into the cytoplasmic membrane of Escherichia coli. Although 3L-Pf3 coat is spontaneously integrated into liposomes composed of phospholipids, diacylglycerol completely blocks such spontaneous integrations at a physiological level. Under the conditions where spontaneous integration does not occur, 3L-Pf3 coat integration was absolutely dependent on a novel integration-stimulating factor. Combination of the PURE system, an in vitro translation system composed of the purified factors involved in translation in E. coli, with liposomes containing the highly purified integration-stimulating factor revealed multiple cycles of 3L-Pf3 coat integration, achieving the complete reconstitution of membrane integration. Based on the function of the factor, we propose that the factor is named MPIase (Membrane Protein Integrase).

本文言語English
ページ(範囲)733-736
ページ数4
ジャーナルBiochemical and Biophysical Research Communications
394
3
DOI
出版ステータスPublished - 2010 4 9
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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