A novel function of synapsin II in neurotransmitter release

Takashi Sugiyama, Toru Shinoe, Yoko Ito, Hidemi Misawa, Takuro Tojima, Etsuro Ito, Tohru Yoshioka*

*この研究の対応する著者

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Although synapsin has been localized to presynaptic structures, its function remains poorly understood. In the present study, we investigated the presynaptic function of synapsin II using a synaptic vesicle recycling process using synapsin-II-overexpressing NG108-15 cells. Western blot analysis with antibodies for synaptic-vesicle-associated protein indicated that the number of synaptic vesicles was approximately doubled in synapsin II transfectants as reported previously. In differentiated synapsin-II-overexpressing and control cells, the application of high potassium induced strong intracellular calcium elevation along neurites and varicosities after differentiation and a weak calcium rise in the cell bodies. The uptake and release of the fluorescent dye FM1-43 revealed that synaptic vesicle recycling in synapsin-II-transfected cells occurred with the same kinetics in the cell body and neuritic varicosities. Furthermore, the area labeled with FM1-43 fluorescence in the synapsin-II-transfected cells was approximately twice as much as in control cells after stimulation, and ATP released after synaptic vesicle fusion with the plasma membrane in synapsin-II-expressing cells was significantly elevated relative to controls. The number of synaptic vesicles paralleled the amount of transmitter released from the cells leading to the conclusion that the number of releasable synaptic vesicles were increased by synapsin II transfection into NG108-15 cells, suggesting that synapsin II may have a role in the regulation of synaptic vesicle number in presynapse-like structures in NG108-15 cells.

本文言語English
ページ(範囲)133-143
ページ数11
ジャーナルMolecular Brain Research
85
1-2
DOI
出版ステータスPublished - 2000 12 28
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 細胞および分子神経科学

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