A novel gene, pmgA, specifically regulates photosystem stoichiometry in the cyanobacterium Synechocystis species PCC 6803 in response to high light

Yukako Hihara, Kintake Sonoike, Masahiko Ikeuchi

研究成果: Article

85 引用 (Scopus)

抄録

Previously, we identified a novel gene, pmgA, as an essential factor to support photomixotrophic growth of Synechocystis species PCC 6803 and reported that a strain in which pmgA was deleted grew better than the wild type under photoautotrophic conditions. To gain insight into the role of pmgA, we investigated the mutant phenotype of pmgA in detail. When low-light-grown (20 μE m-2 s-1) cells were transferred to high light (HL [200μE m-2 s-1]), pmgA mutants failed to respond in the manner typically associated with Synechocystis. Specifically, mutants lost their ability to suppress accumulation of chlorophyll and photosystem I and, consequently, could not modulate photosystem stoichiometry. These phenotypes seem to result in enhanced rates of photosynthesis and growth during short-term exposure to HL. Moreover, mixed-culture experiments clearly demonstrated that loss of pmgA function was selected against during longer-term exposure to HL, suggesting that pmgA is involved in acquisition of resistance to HL stress. Finally, early induction of pmgA expression detected by reverse transcriptase-PCR upon the shift to HL led us to conclude that pmgA is the first gene identified, to our knowledge, as a specific regulatory factor for H L acclimation.

元の言語English
ページ(範囲)1205-1216
ページ数12
ジャーナルPlant Physiology
117
発行部数4
出版物ステータスPublished - 1998
外部発表Yes

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Synechocystis
Cyanobacteria
stoichiometry
Phenotype
Photosystem I Protein Complex
Complement Factor H
Light
mutants
Acclimatization
Photosynthesis
Chlorophyll
Growth
Reverse Transcriptase Polymerase Chain Reaction
Genes
phenotype
genes
photosystem I
mixed culture
chronic exposure
acclimation

ASJC Scopus subject areas

  • Plant Science

これを引用

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abstract = "Previously, we identified a novel gene, pmgA, as an essential factor to support photomixotrophic growth of Synechocystis species PCC 6803 and reported that a strain in which pmgA was deleted grew better than the wild type under photoautotrophic conditions. To gain insight into the role of pmgA, we investigated the mutant phenotype of pmgA in detail. When low-light-grown (20 μE m-2 s-1) cells were transferred to high light (HL [200μE m-2 s-1]), pmgA mutants failed to respond in the manner typically associated with Synechocystis. Specifically, mutants lost their ability to suppress accumulation of chlorophyll and photosystem I and, consequently, could not modulate photosystem stoichiometry. These phenotypes seem to result in enhanced rates of photosynthesis and growth during short-term exposure to HL. Moreover, mixed-culture experiments clearly demonstrated that loss of pmgA function was selected against during longer-term exposure to HL, suggesting that pmgA is involved in acquisition of resistance to HL stress. Finally, early induction of pmgA expression detected by reverse transcriptase-PCR upon the shift to HL led us to conclude that pmgA is the first gene identified, to our knowledge, as a specific regulatory factor for H L acclimation.",
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AU - Sonoike, Kintake

AU - Ikeuchi, Masahiko

PY - 1998

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