A Novel Modified Nucleoside Found at the First Position of the Anticodon of Methionine tRNA from Bovine Liver Mitochondria

Jiro Moriya, Takashi Yokogawa, Kawori Wakita, Takuya Ueda, Kazuya Nishikawa, Pamela F. Crain, Takeshi Hashizume, Steven C. Pomerantz, James A. McCloskey, Gota Kawai, Nobuhiro Hayashi, Shigeyuki Yokoyama, Kimitsuna Watanabe*

*この研究の対応する著者

研究成果: Article査読

80 被引用数 (Scopus)

抄録

Methionine tRNA was purified from bovine liver mitochondria, and its nucleotide sequence was determined. The tRNA possesses only three posttranscriptionally modified nucleosides, two pseudouridines in the anticodon and T stems and a previously unknown nucleoside specified by the gene sequence as cytidine, in the first position of the anticodon. Structure analysis of the anticodon nucleoside by mass spectrometry revealed a molecular mass 28 Da greater than that of cytidine, and unmodified ribose, with substitution at C-5 implied by hydrogen–deuterium exchange experiments. Proton NMR of the intact tRNA showed presence of a formyl moiety, thus leading to the candidate structure 5-formylcytidine (f5C), not a previously known compound. The structure assignment was confirmed by chemical synthesis and comparison of data from combined HPLC/mass spectrometry and proton NMR for the natural and synthetic nucleosides. The potential function of f5C in the tRNAMet anticodon is discussed with regard to codon-anticodon interactions.

本文言語English
ページ(範囲)2234-2239
ページ数6
ジャーナルBiochemistry
33
8
DOI
出版ステータスPublished - 1994 3 1
外部発表はい

ASJC Scopus subject areas

  • 生化学

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