A novel rat hypothalamic RFamide-related peptide identified by immunoaffinity chromatography and mass spectrometry

Kazuyoshi Ukena*, Eiko Iwakoshi, Hiroyuki Minakata, Kazuyoshi Tsutsui

*この研究の対応する著者

研究成果: Article査読

159 被引用数 (Scopus)

抄録

Recently, cDNAs encoding novel RFamide-related peptides (RFRPs) have been reported in the mammalian brains by a gene database search and the deduced RFRPs have been suggested to participate in neuroendocrine and pain mechanisms in the rat. Two peptides have been predicted to be encoded in the cDNA of rodent RFRPs. To assess precise functions of rodent RFRPs in the brain, in the present study we identified a naturally occurring RFRP in the rat hypothalamus by immunoaffinity purification combined with mass spectrometry (MS). The affinity chromatography showed that the rat hypothalamus contained RFRP-like immunoreactivity. The immunoreactive material was analyzed by a nanoflow electrospray ionization time-of-flight MS followed by tandem MS analysis. The mass peak corresponding to octadecapeptide was detected at 1010.54 m/z ([M+2H]2+) and its sequence, ANMEAGTMSHFPSLPQRF-NH2, was revealed by the fragmentation, showing a mature form encoded in the cDNA sequence of RFRPs. The identified endogenous RFRP will aid not only in defining its physiological roles but also facilitate the development of its agonists and antagonists in the rodent brain.

本文言語English
ページ(範囲)255-258
ページ数4
ジャーナルFEBS Letters
512
1-3
DOI
出版ステータスPublished - 2002 2月 13
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生物理学
  • 分子生物学

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