A novel system for constructing a recombinant highly-attenuated Vaccinia virus strain (LC16m8) expressing foreign genes and its application for the generation of LC16m8-based vaccines against herpes simplex virus 2

Natsumi Omura, Tomoki Yoshikawa, Hikaru Fujii, Miho Shibamura, Takuya Inagaki, Hirofumi Kato, Kazutaka Egawa, Shizuko Harada, Souichi Yamada, Haruko Takeyama, Masayuki Saijo

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    A novel system was developed for generating highly attenuated vaccinia virus LC16m8 (m8, third-generation smallpox vaccine) that expresses foreign genes. The innovations in this system are its excisable selection marker, specificity of the integration site of a gene of interest, and easy identification of clones with a fluorescent signal. Using this system, recombinant m8s, which expressed herpes simplex virus 2 (HSV-2) glycoprotein B (gB)-, gD-, or both gB and gD (gB + gD), were generated, and their efficacy was evaluated. First, the induction of a specific IgG against these HSV-2 glycoproteins in mice infected with one of these recombinant m8s was confirmed by an immunofluorescent assay. Next, mice preinfected with one of the recombinant m8s were infected with HSV-2 at a lethal dose to examine the vaccine efficacy. The fatality rate among the mice preinfected with either the recombinant gB + gD- or gD-expressing m8 significantly decreased in comparison with the control. The survival rate in male and female mice preinfected with either the recombinant gB + gD- or gD-expressing m8 increased to 100% and 60%, respectively, while most of the control mice died. In summary, this new system may be applicable to creation of a novel m8-based vaccine.

    元の言語English
    ページ(範囲)229-233
    ページ数5
    ジャーナルJapanese Journal of Infectious Diseases
    71
    発行部数3
    DOI
    出版物ステータスPublished - 2018 1 1

    ASJC Scopus subject areas

    • Microbiology (medical)
    • Infectious Diseases

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