A nucleoside kinase as a dual selector for genetic switches and circuits

Yohei Tashiro, Hiroki Fukutomi, Kei Terakubo, Kyoichi Saito, Daisuke Umeno*

*この研究の対応する著者

研究成果: Article査読

30 被引用数 (Scopus)

抄録

The development of genetic switches and their integrated forms (genetic circuits) with desired specifications/functions is key for success in synthetic biology. Due to the difficulty in rational design, genetic switches and circuits with desirable specifications are mostly obtained by directed evolution. Based on a virus-derived nucleotide kinase as a single-gene dual selector, we constructed a robust, efficient and stringent selection system for genetic switches. This method exhibited unprecedented enrichment efficacy (>30000-fold) of functional switches from non-functional ones in a single selection cycle. In addition, negative (OFF) selection was exceptionally stringent, allowing the rapid and efficient selection of non-leaky from leaky circuits.

本文言語English
ページ(範囲)e12
ジャーナルNucleic acids research
39
3
DOI
出版ステータスPublished - 2011 2月
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

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