抄録
It is known that acute physical exercise may have diverse pathophysiological consequences in various organs due to free radical formation. We have investigated whether a period of anaerobic running to exhaustion in rats results in oxidative modification of proteins in the lungs. Six rats of an exercised group (E) ran for two periods of 5 min at a speed of 30 m·min-1 followed by a recovery period of 5 min, and then by a third period of running to exhaustion. Reactive carbonyl derivatives (RCD) were measured by the Western blot technique on lungs of E and control (C) rats. In addition, the activity of glutamine synthetase (GS) was also monitored as marker of oxidative damage to proteins. This investigation revealed significant exercise-induced increases in accumulation of RCD in the lungs of the E group compared with the C group. The RCD signals were visibly stronger in proteins with molecular weight of 55 kDa and 32 kDa. The activity of GS was higher by about 30% in E rats than in C rats. The present data suggest that anaerobic exercise induces protein oxidation in the lungs.
本文言語 | English |
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ページ(範囲) | 439-441 |
ページ数 | 3 |
ジャーナル | Pflugers Archiv European Journal of Physiology |
巻 | 435 |
号 | 3 |
DOI | |
出版ステータス | Published - 1998 2月 18 |
外部発表 | はい |
ASJC Scopus subject areas
- 生理学
- 臨床生化学
- 生理学(医学)