A reduction-triggered fluorescence probe for sensing nucleic acids

Hiroshi Abe, Jin Wang, Kazuhiro Furukawa, Kazuma Oki, Miwako Uda, Satoshi Tsuneda, Yoshihiro Ito

    研究成果: Article

    78 引用 (Scopus)

    抄録

    We have developed a reduction-triggered fluorescence probe with a new fluorogenic compound derivatized from Rhodamine for sensing oligonucleotides. The chemistry to activate the compound involves the reaction between the azide group of rhodamine derivatives and the reducing reagents, with the fluorescence signal appearing after reduction of the azide group. The signal/background ratio of this fluorogenic compound reached 2100-fold enhancement in fluorescence intensity. Dithio-1,4-threitol or triphenylphosphine as reducing reagents were successfully utilized for this chemistry to be introduced into the DNA probe. The genetic detection requires that two strands of DNA bind onto target oligonucleotides, one probe carrying a reducible fluorogenic compound while the other carries the reducing reagents. The reaction proceeds automatically without any enzymes or reagents under biological conditions to produce a fluorescence signal within 10-20 min in the presence of target DNA or RNA. In addition, the probe was very stable under biological conditions, even such extreme conditions as pH 5 solution, pH 10 solution, or high temperature (90°C) with no undesirable background signal. The probes were successfully applied to the detection of oligonucleotides at the single nucleotide level in solution and endogenous RNA in bacterial cells.

    元の言語English
    ページ(範囲)1219-1226
    ページ数8
    ジャーナルBioconjugate Chemistry
    19
    発行部数6
    DOI
    出版物ステータスPublished - 2008 6

    Fingerprint

    Nucleic Acid Probes
    Nucleic acids
    Nucleic Acids
    Fluorescence
    Oligonucleotides
    Rhodamines
    Azides
    DNA
    RNA
    Bacterial RNA
    Oligonucleotide Probes
    DNA Probes
    Nucleotides
    Derivatives
    Enzymes
    Temperature

    ASJC Scopus subject areas

    • Chemistry(all)
    • Organic Chemistry
    • Clinical Biochemistry
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry

    これを引用

    Abe, H., Wang, J., Furukawa, K., Oki, K., Uda, M., Tsuneda, S., & Ito, Y. (2008). A reduction-triggered fluorescence probe for sensing nucleic acids. Bioconjugate Chemistry, 19(6), 1219-1226. https://doi.org/10.1021/bc800014d

    A reduction-triggered fluorescence probe for sensing nucleic acids. / Abe, Hiroshi; Wang, Jin; Furukawa, Kazuhiro; Oki, Kazuma; Uda, Miwako; Tsuneda, Satoshi; Ito, Yoshihiro.

    :: Bioconjugate Chemistry, 巻 19, 番号 6, 06.2008, p. 1219-1226.

    研究成果: Article

    Abe, H, Wang, J, Furukawa, K, Oki, K, Uda, M, Tsuneda, S & Ito, Y 2008, 'A reduction-triggered fluorescence probe for sensing nucleic acids', Bioconjugate Chemistry, 巻. 19, 番号 6, pp. 1219-1226. https://doi.org/10.1021/bc800014d
    Abe, Hiroshi ; Wang, Jin ; Furukawa, Kazuhiro ; Oki, Kazuma ; Uda, Miwako ; Tsuneda, Satoshi ; Ito, Yoshihiro. / A reduction-triggered fluorescence probe for sensing nucleic acids. :: Bioconjugate Chemistry. 2008 ; 巻 19, 番号 6. pp. 1219-1226.
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