A simplified method for the preparation of transcriptionally active liver nuclear extracts

Masahira Hattori, A. Tugores, L. Veloz, M. Karin, D. A. Brenner

研究成果: Article

110 引用 (Scopus)

抜粋

We have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts.

元の言語English
ページ(範囲)777-781
ページ数5
ジャーナルDNA and Cell Biology
9
発行部数10
出版物ステータスPublished - 1990
外部発表Yes

    フィンガープリント

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

これを引用

Hattori, M., Tugores, A., Veloz, L., Karin, M., & Brenner, D. A. (1990). A simplified method for the preparation of transcriptionally active liver nuclear extracts. DNA and Cell Biology, 9(10), 777-781.