A simplified method for the preparation of transcriptionally active liver nuclear extracts

Masahira Hattori, A. Tugores, L. Veloz, M. Karin, D. A. Brenner

研究成果: Article査読

113 被引用数 (Scopus)

抄録

We have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts.

本文言語English
ページ(範囲)777-781
ページ数5
ジャーナルDNA and Cell Biology
9
10
出版ステータスPublished - 1990
外部発表はい

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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