A soft, stretchable and conductive biointerface for cell mechanobiology

Irene Bernardeschi, Francesco Greco, Gianni Ciofani, Attilio Marino, Virgilio Mattoli, Barbara Mazzolai, Lucia Beccai

研究成果: Article査読

13 被引用数 (Scopus)

抄録

In mechanobiology the study of cell response to mechanical stimuli is fundamental, and the involved processes (i.e., mechanotransduction) need to be investigated by interfacing (mechanically and electrically) with the cells in dynamic and non-invasive natural-like conditions. In this work, we present a novel soft, stretchable and conductive biointerface that allows both cell mechanical stimulation and dynamic impedance recording. The biointerface stretchability and conductivity, jointly to the biocompatibility and transparency needed to perform cell culture studies, were obtained by exploiting the formation of wrinkles on the surface of a 90 nm thick conductive layer of poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) on a pre-stretched 130 μm thick poly(dimethylsiloxane) (PDMS) substrate. Cell adhesion and proliferation of SH-SY5Y human neuroblastoma cells were evaluated, and cell differentiation on the corrugated surface was assessed. We demonstrate how the biointerface remains conductive when applying uniaxial strain up to 10 %, and when cell culturing is performed. Finally, a reduction of about 30 % of the relative impedance variation signal was measured, with respect to the control, as a result of the mechanical stimulation of cells.

本文言語English
ジャーナルBiomedical Microdevices
17
2
DOI
出版ステータスPublished - 2015 4 1
外部発表はい

ASJC Scopus subject areas

  • 生体医工学
  • 分子生物学
  • 医学(全般)

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