A Strategy for Designing Thermostable Enzymes by Reconstructing Ancestral Sequences Possessed by Ancient Life

Satoshi Akanuma, Akihiko Yamagishi*

*この研究の対応する著者

研究成果: Chapter

3 被引用数 (Scopus)

抄録

The amino acid sequences of some ancestral proteins have been inherited in their descendants with a few modifications. Therefore, in some cases, a phylogenetic tree can be constructed by comparing extant amino acid sequences that were evolved from a single common ancestor. Moreover, ancestral sequences of a particular protein can be inferred using the topology of the phylogenetic tree in combination with the extant amino acid sequences contained in the tree. We recently inferred ancestral amino acid sequences of nucleoside diphosphate kinase that might have existed 3,500–3,800 million years ago. Physicochemical analysis of the experimentally reconstructed ancestral sequences revealed that the resurrected proteins are stable around 100 °C. Given the hyperthermophilicity of ancient organisms, the ancestral sequence reconstruction technique presented in this chapter will serve as a generic method for creating thermally stable proteins.

本文言語English
ホスト出版物のタイトルGrand Challenges in Biology and Biotechnology
出版社Springer Science and Business Media B.V.
ページ581-596
ページ数16
DOI
出版ステータスPublished - 2016

出版物シリーズ

名前Grand Challenges in Biology and Biotechnology
1
ISSN(印刷版)2367-1017
ISSN(電子版)2367-1025

ASJC Scopus subject areas

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー
  • バイオエンジニアリング

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