A Tetraspanin-Family Protein, T-Cell Acute Lymphoblastic Leukemia-Associated Antigen 1, Is Induced by the Ewing's Sarcoma-Wilms' Tumor 1 Fusion Protein of Desmoplastic Small Round-Cell Tumor

Emi Ito, Reiko Honma, Jun Ichi Imai, Sakura Azuma, Takayuki Kanno, Shigeo Mori, Osamu Yoshie, Jun Nishio, Hiroshi Iwasaki, Koichi Yoshida, Jin Gohda, Jun Ichiro Inoue, Shinya Watanabe, Kentaro Senba

研究成果: Article

36 引用 (Scopus)

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Recurrent chromosomal translocations in neoplasms often generate hybrid genes that play critical roles in tumorigenesis. Desmoplastic small round-cell tumor (DSRCT) is an aggressive malignancy associated with the chromosomal translocation t(11;22)(p13;q12). This translocation generates a chimeric transcription factor, EWS-WT1, which consists of the transcriptional activation domain of the Ewing's sarcoma (EWS) protein and the DNA binding domain of the Wilms' tumor 1 (WT1) protein. One of the splice variants, EWS-WT1(-KTS) lacks three amino acid residues (Lys-Thr-Ser) in the DNA binding domain and transforms NIH3T3 cells. Therefore, it is likely that aberrant gene expression caused by EWS-WT1(-KTS) is involved in the malignant phenotype of DSRCT. Microarray analysis of 9600 human genes revealed that a gene encoding a tetraspanin-family protein, T-cell acute lymphoblastic leukemia-associated antigen 1 (TALLA-1), was induced in EWS-WT1(-KTS)-expressing cell clones. This induction was EWS-WT1(-KTS)-specific, and more importantly, TALLA-1 protein was expressed in the three independent cases of DSRCT. Tetraspanin-family genes encode transmembrane proteins that regulate various cell processes such as cell adhesion, migration and metastasis. Our findings provide a novel insight into the malignant phenotype of DSRCT, suggesting that TALLA-1 is a useful marker for diagnosis and a potential target for the therapy of DSRCT.

元の言語English
ページ(範囲)2165-2172
ページ数8
ジャーナルAmerican Journal of Pathology
163
発行部数6
出版物ステータスPublished - 2003 12
外部発表Yes

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine

これを引用

Ito, E., Honma, R., Imai, J. I., Azuma, S., Kanno, T., Mori, S., Yoshie, O., Nishio, J., Iwasaki, H., Yoshida, K., Gohda, J., Inoue, J. I., Watanabe, S., & Senba, K. (2003). A Tetraspanin-Family Protein, T-Cell Acute Lymphoblastic Leukemia-Associated Antigen 1, Is Induced by the Ewing's Sarcoma-Wilms' Tumor 1 Fusion Protein of Desmoplastic Small Round-Cell Tumor. American Journal of Pathology, 163(6), 2165-2172.