AAU-specific RNA cleavage mediated by MazF toxin endoribonuclease conserved in Nitrosomonas Europaea

Tatsuki Miyamoto, Akiko Yokota, Satoshi Tsuneda, Naohiro Noda

研究成果: Article

9 引用 (Scopus)

抜粋

Nitrosomonas europaea carries numerous toxin-antitoxin systems. However, despite the abundant representation in its chromosome, studies have not surveyed the underlying molecular functions in detail, and their biological roles remain enigmatic. In the present study, we found that a chromosomally-encoded MazF family member, predicted at the locus NE1181, is a functional toxin endoribonuclease, and constitutes a toxin-antitoxin system, together with its cognate antitoxin, MazE. Massive parallel sequencing provided strong evidence that this toxin endoribonuclease exhibits RNA cleavage activity, primarily against the AAU triplet. This sequence-specificity was supported by the results of fluorometric assays. Our results indicate that N. europaea alters the translation profile and regulates its growth using the MazF family of endoribonuclease under certain stressful conditions.

元の言語English
記事番号174
ジャーナルToxins
8
発行部数6
DOI
出版物ステータスPublished - 2016 6

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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