AAU-specific RNA cleavage mediated by MazF toxin endoribonuclease conserved in Nitrosomonas Europaea

Tatsuki Miyamoto, Akiko Yokota, Satoshi Tsuneda*, Naohiro Noda

*この研究の対応する著者

研究成果: Article査読

9 被引用数 (Scopus)

抄録

Nitrosomonas europaea carries numerous toxin-antitoxin systems. However, despite the abundant representation in its chromosome, studies have not surveyed the underlying molecular functions in detail, and their biological roles remain enigmatic. In the present study, we found that a chromosomally-encoded MazF family member, predicted at the locus NE1181, is a functional toxin endoribonuclease, and constitutes a toxin-antitoxin system, together with its cognate antitoxin, MazE. Massive parallel sequencing provided strong evidence that this toxin endoribonuclease exhibits RNA cleavage activity, primarily against the AAU triplet. This sequence-specificity was supported by the results of fluorometric assays. Our results indicate that N. europaea alters the translation profile and regulates its growth using the MazF family of endoribonuclease under certain stressful conditions.

本文言語English
論文番号174
ジャーナルToxins
8
6
DOI
出版ステータスPublished - 2016 6

ASJC Scopus subject areas

  • 毒物学
  • 健康、毒物学および変異誘発

フィンガープリント

「AAU-specific RNA cleavage mediated by MazF toxin endoribonuclease conserved in Nitrosomonas Europaea」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル