Accelerated evolution in the protein-coding regions is universal in crotalinae snake venom gland phospholipase A2 isozyme genes

Kin Ichi Nakashima, Ikuo Nobuhisa, Masanobu Deshimaru, Makoto Nakai, Tomohisa Ogawa, Yasuyuki Shimohigashi, Yasuyuki Fukumaki, Masahira Hattori, Yoshiyuki Sakaki, Shosaku Hattori, Motonori Ohno

研究成果: Article

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The nucleotide sequences of four genes encoding Trimeresurus gramineus (green habu snake, crotalinae) venom gland phospholipase A2 (PLA2; phosphatidylcholine 2-acylhydrolase, EC 3.1.1.4) isozymes were compared internally and externally with those of six genes encoding Trimeresurus flavoviridis (habu snake, crotalinae) venom gland PLA2 isozymes. The numbers of nucleotide substitutions per site (K(N)) for the noncoding regions including introns were one-third to one-eighth of the numbers of nucleotide substitutions per synonymous site (K(S)) for the protein-coding regions of exons, indicating that the noncoding regions are much more conserved than the protein-coding regions. The K(N) values for the introns were found to be nearly equivalent to those of introns of T. gramineus and T. flavoviridis TATA box-binding protein genes, which are assumed to be a general (nonvenomous) gene. Thus, it is evident that the introns of venom gland PLA2 isozyme genes have evolved at a similar rate to those of nonvenomous genes. The numbers of nucleotide substitutions per nonsynonymous site (K(A)) were close to or larger than the K(S) values for the protein-coding regions in venom gland PLA2 isozyme genes. All of the data combined reveal that Darwinian-type accelerated evolution has universally occurred only in the protein-coding regions of crotalinae snake venom PLA2 isozyme genes.

元の言語English
ページ(範囲)5605-5609
ページ数5
ジャーナルProceedings of the National Academy of Sciences of the United States of America
92
発行部数12
DOI
出版物ステータスPublished - 1995 6 6
外部発表Yes

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ASJC Scopus subject areas

  • Genetics
  • General

これを引用

Nakashima, K. I., Nobuhisa, I., Deshimaru, M., Nakai, M., Ogawa, T., Shimohigashi, Y., Fukumaki, Y., Hattori, M., Sakaki, Y., Hattori, S., & Ohno, M. (1995). Accelerated evolution in the protein-coding regions is universal in crotalinae snake venom gland phospholipase A2 isozyme genes. Proceedings of the National Academy of Sciences of the United States of America, 92(12), 5605-5609. https://doi.org/10.1073/pnas.92.12.5605