Accelerated evolution of Trimeresurus flavoviridis venom gland phospholipase A2 isozymes

Kin Ichi Nakashima, Tomohisa Ogawa, Naoko Oda, Masahira Hattori, Yoshiyuki Sakaki, Hiroshi Kihara, Motonori Ohno*

*この研究の対応する著者

研究成果: Article査読

159 被引用数 (Scopus)

抄録

Six Trimeresurus flavoviridis (Habu snake) venom gland phospholipase A2 (PLA2) isozyme genes were found to consist of four exons and three introns and to encode proteins of 138 amino acid residues, including the signal sequence of 16 amino acid residues. Comparison of the nucleotide sequences showed that the introns are much more homologous than the protein-coding regions of exons except for the signal peptide-coding region of the first exon. The numbers of nucleotide substitutions per site (AN) for introns are approximately one-fourth of the numbers of nucleotide substitutions per synonymous site (KS) for the protein-coding regions, indicating that the introns are unusually conserved. The absence of an apparent functional role for the introns suggests that the protein-coding regions, except for the signal peptide-coding domains, have evolved at greater substitution rates than introns. The fact that the numbers of nucleotide substitutions per nonsynonymous site (KA) are close to or larger than KS values for relevant pairs of genes revealed that Darwinian-type accelerated substitutions have occurred in the protein-coding regions or exons. This is compatible with the presence of PLA2 species with diverse physiological activities in the venom.

本文言語English
ページ(範囲)5964-5968
ページ数5
ジャーナルProceedings of the National Academy of Sciences of the United States of America
90
13
出版ステータスPublished - 1993 7 1
外部発表はい

ASJC Scopus subject areas

  • 一般
  • 遺伝学

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