@article{55fdec989f314659b9f680e919f14d17,
title = "Accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells",
abstract = "At the initial stage of carcinogenesis, transformation occurs in single cells within the epithelium. Recent studies have revealed that the newly emerging transformed cells are often apically eliminated from epithelial tissues. However, the underlying molecular mechanisms of this cancer preventive phenomenon still remain elusive. In this study, we first demonstrate that myosin-II accumulates in Src-transformed cells when they are surrounded by normal epithelial cells. Knock-down of the heavy chains of myosin-II substantially diminishes apical extrusion of Src cells, suggesting that accumulated myosin-II positively regulates the apical elimination of transformed cells. Furthermore, we have identified β-spectrin as a myosin-II-binding protein under the coculture of normal and Src-transformed epithelial cells. β-spectrin is also accumulated in Src cells that are surrounded by normal cells, and the β-spectrin accumulation is regulated by myosin-II. Moreover, knock-down of β-spectrin significantly suppresses apical extrusion of Src cells. Collectively, these results indicate that accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells. Further elucidation of the molecular mechanisms of apical extrusion would lead to the establishment of a novel type of cancer preventive medicine.",
author = "Mikio Takagi and Masaya Ikegawa and Takashi Shimada and Susumu Ishikawa and Mihoko Kajita and Takeshi Maruyama and Tomoko Kamasaki and Yasuyuki Fujita",
note = "Funding Information: This study was supported by Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research on Innovative Areas 26114001, Grant-in-Aid for Scientific Research (A) 18H03994, Strategic Japanese-Swiss Science and Technology Program, AMED under Grant Number JP17ck0106361 and JP18cm0106234, SAN-ESU GIKEN CO. LTD, the Naito Foundation, and the Takeda Science Foundation. Funding Information: This study was supported by Japan Society for the Promotion of Science (JSPS) Grant‐in‐Aid for Scientific Research on Innovative Areas 26114001, Grant‐in‐Aid for Scientific Research (A) 18H03994, Strategic Japanese‐Swiss Science and Technology Program, AMED under Grant Number JP17ck0106361 and JP18cm0106234, SAN‐ESU GIKEN CO. LTD, the Naito Foundation, and the Takeda Science Foundation. Funding Information: Japan Society for the Promotion of Science, Grant/Award Number: 26114001, and 18H03994,; Strategic Japanese‐Swiss Science and Technology Program, AMED, Grant/Award Number: JP17ck0106361 and JP18cm0106234; SAN‐ESU GIKEN CO. LTD; Naito Foundation; Takeda Science Foundation Publisher Copyright: {\textcopyright} 2018 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd",
year = "2018",
month = nov,
doi = "10.1111/gtc.12643",
language = "English",
volume = "23",
pages = "974--981",
journal = "Genes to Cells",
issn = "1356-9597",
publisher = "Wiley-Blackwell",
number = "11",
}