Actin cytoskeleton is responsible for the change of cytoplasmic organization in root hair cells induced by a protein phosphatase inhibitor, calyculin A

E. Yokota*, N. Imamichi, M. Tominaga, T. Shimmen

*この研究の対応する著者

研究成果: Article査読

13 被引用数 (Scopus)

抄録

In root hair cells of Limnobium stoloniferum, a protein phosphatase inhibitor, calyculin A (CA), at concentrations higher than 50 nM inhibits cytoplasmic streaming and induces remarkable morphological changes in the cytoplasm: the transvacuolar strands disperse and spherical cytoplasmic bodies emerge. The mechanism of the morphological changes of the cytoplasm induced by CA was studied by pharmacological analyses. The formation of spherical bodies in cells treated with CA was suppressed by the actin-depolymerizing and -fragmenting drugs latrunculin B and cytochalasin D at concentrations higher than 100 nM and 5 μM, respectively. In contrast, 100 μM propyzamide, a microtubule-depolymerizing drug, did not affect the formation of spherical bodies by CA. Interestingly, 60 mM 2,3-butanedione monoxime, an inhibitor of myosin, also suppressed the CA-induced formation of cytoplasmic spherical bodies. These results indicate that the actin cytoskeleton is intimately involved in the morphological changes of the cytoplasm induced by CA.

本文言語English
ページ(範囲)184-193
ページ数10
ジャーナルProtoplasma
213
3-4
DOI
出版ステータスPublished - 2000
外部発表はい

ASJC Scopus subject areas

  • 植物科学
  • 細胞生物学

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