Actinomadura barringtoniae sp. Nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn

Hathairat Rachniyom, Atsuko Matsumoto, Yuki Inahashi, Akira Take, Yoko Takahashi, Arinthip Thamchaipenet

研究成果: Article

3 引用 (Scopus)

抄録

A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.] at Khao Khitchakut district, Chantaburi province, Thailand, was characterized by using a polyphasic approach. The strain formed a branched substrate and aerial mycelia which differentiated into straight to flexuous chains of smoothornamented spores. Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan. The whole-cell sugars were glucose, madurose, mannose, rhamnose and ribose. Mycolic acids were absent. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside. The predominant menaquinones were MK-9(H6), MK-9(H8), MK-9(H0) and MK-9(H4). The major fatty acids were C16: 0, C18: 1ω9c and 10-methyl C18: 0 (tuberculostearic acid). The genomic DNA G+C content was 70.5 mol%. Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2% sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %). The levels of DNA–DNA relatedness between strain GKU 128T and the closely related type species were less than 19 %. On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp. nov. (=TBRC 7225T=NBRC 113074T) is proposed.

元の言語English
記事番号002714
ページ(範囲)1584-1590
ページ数7
ジャーナルInternational Journal of Systematic and Evolutionary Microbiology
68
発行部数5
DOI
出版物ステータスPublished - 2018 5 1
外部発表Yes

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Barringtonia
Actinomadura
Mycolic Acids
Diaminopimelic Acid
Quercus
Vitamin K 2
Rhamnose
Phosphatidylglycerols
Cardiolipins
Ribose
Peptidoglycan
Actinobacteria
Mycelium
Base Composition
Thailand
Mannose
Phosphatidylinositols
Spores
rRNA Genes
Cell Wall

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

これを引用

Actinomadura barringtoniae sp. Nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn. / Rachniyom, Hathairat; Matsumoto, Atsuko; Inahashi, Yuki; Take, Akira; Takahashi, Yoko; Thamchaipenet, Arinthip.

:: International Journal of Systematic and Evolutionary Microbiology, 巻 68, 番号 5, 002714, 01.05.2018, p. 1584-1590.

研究成果: Article

Rachniyom, Hathairat ; Matsumoto, Atsuko ; Inahashi, Yuki ; Take, Akira ; Takahashi, Yoko ; Thamchaipenet, Arinthip. / Actinomadura barringtoniae sp. Nov., an endophytic actinomycete isolated from the roots of Barringtonia acutangula (L.) Gaertn. :: International Journal of Systematic and Evolutionary Microbiology. 2018 ; 巻 68, 番号 5. pp. 1584-1590.
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abstract = "A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.] at Khao Khitchakut district, Chantaburi province, Thailand, was characterized by using a polyphasic approach. The strain formed a branched substrate and aerial mycelia which differentiated into straight to flexuous chains of smoothornamented spores. Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan. The whole-cell sugars were glucose, madurose, mannose, rhamnose and ribose. Mycolic acids were absent. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside. The predominant menaquinones were MK-9(H6), MK-9(H8), MK-9(H0) and MK-9(H4). The major fatty acids were C16: 0, C18: 1ω9c and 10-methyl C18: 0 (tuberculostearic acid). The genomic DNA G+C content was 70.5 mol{\%}. Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2{\%} sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 {\%}). The levels of DNA–DNA relatedness between strain GKU 128T and the closely related type species were less than 19 {\%}. On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp. nov. (=TBRC 7225T=NBRC 113074T) is proposed.",
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AU - Take, Akira

AU - Takahashi, Yoko

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AB - A novel actinomycete strain, designated GKU 128T, isolated from the roots of an Indian oak tree [Barringtonia acutangula (L.) Gaertn.] at Khao Khitchakut district, Chantaburi province, Thailand, was characterized by using a polyphasic approach. The strain formed a branched substrate and aerial mycelia which differentiated into straight to flexuous chains of smoothornamented spores. Analysis of the cell wall revealed the presence of meso-diaminopimelic acid and N-acetylmuramic acid in the peptidoglycan. The whole-cell sugars were glucose, madurose, mannose, rhamnose and ribose. Mycolic acids were absent. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannoside. The predominant menaquinones were MK-9(H6), MK-9(H8), MK-9(H0) and MK-9(H4). The major fatty acids were C16: 0, C18: 1ω9c and 10-methyl C18: 0 (tuberculostearic acid). The genomic DNA G+C content was 70.5 mol%. Based on 16S rRNA gene sequence analysis, strain GKU 128T was closely related to the type strains of Actinomadura nitritigenes NBRC 15918T (99.2% sequence similarity) and Actinomadura fibrosa JCM 9371T (98.7 %). The levels of DNA–DNA relatedness between strain GKU 128T and the closely related type species were less than 19 %. On the basis of phenotypic and genotypic characteristics, strain GKU 128T could be distinguished from its closely related type strains and represents a novel species of the genus Actinomadura, for which the name Actinomadura barringtoniae sp. nov. (=TBRC 7225T=NBRC 113074T) is proposed.

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KW - Endophytic actinomycete

KW - Indian oak tree

KW - New species

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